Evidence map›Paper›PMID 41254365›Full record

ArticleNature methods2025

Light-induced extracellular vesicle and particle adsorption.

Colin L Hisey, Xilal Y Rima, Jacob Doon-Ralls, Chiranth K Nagaraj, Sophia Mayone, Kim Truc Nguyen, Sydney Wiggins, Kalpana Deepa Priya Dorayappan, Xin Huang, Mangesh D Hade and 12 more

Abstract read
In one paragraph

Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

Colin L Hisey *Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0000-0001-8732-3600
Xilal Y Rima *Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0000-0001-5633-5887
Jacob Doon-Ralls *Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Chiranth K NagarajDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Sophia MayoneDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Kim Truc NguyenDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Sydney WigginsDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0009-0007-6039-8291
Kalpana Deepa Priya DorayappanComprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
Xin HuangDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Mangesh D HadeDepartment of Pediatrics, Center for Clinical and Translational Research, Nationwide Children's Hospital, Columbus, OH, USA.ORCID http://orcid.org/0000-0002-5937-9049
Karuppaiyah SelvendiranComprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
James N HigginbothamDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Oleg TutanovDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Jeffrey L FranklinDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Robert J CoffeyDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.ORCID http://orcid.org/0000-0002-2180-3844
David WoodDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Chunyu HuDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Divya S PatelDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0009-0005-7766-2111
Setty M MagañaDepartment of Pediatrics, Center for Clinical and Translational Research, Nationwide Children's Hospital, Columbus, OH, USA.
Andre F PalmerDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0000-0002-3378-3772
Derek HansfordDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0000-0002-0483-2715
Eduardo ReáteguiDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA. reategui.8@osu.edu.ORCID http://orcid.org/0000-0002-8271-8205

Funding

Vanderbilt-Ingram Cancer Center SPORE in Gastrointestinal CancerP50CA236733 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MARY Kay WASHINGTON · 2019 to 2026
$19.6M
Roles for Supermeres in CRC ProgressionP01CA229123 · NCI · VANDERBILT UNIVERSITY · PI Yu Shyr · 2020 to 2026
$12.9M
Integrated approach to study early and late events in colonic neoplasia: mouse to manR35CA197570 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Robert J. Coffey · 2017 to 2026
$9.4M
Uncovering exRNA and protein determinants of secreted vesicle heterogeneity by flow cytometric purification of vesicle subsets from cells and plasmaUH3CA241685 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI CHAREST, ALAIN, COFFEY, ROBERT J. · 2021 to 2022
$2.1M
Multi-parametric Integrated Molecular Detection of SARS-CoV-2 from Biofluids by Adapting Single Extracellular Vesicle Characterization TechnologiesU18TR003807 · NCATS · OHIO STATE UNIVERSITY · PI LEE, INYOUL, LEE, LY JAMES · 2021 to 2022
$1.8M
Postdoctoral Training in Cardiometabolic ScienceT32HL149637 · NHLBI · OHIO STATE UNIVERSITY · PI HSUEH, WILLA A, SMITH, SAKIMA AHMAD · 2020 to 2024
$1.5M
CTSA Predoctoral T32 at the Ohio State UniversityT32TR004543 · NCATS · OHIO STATE UNIVERSITY · PI GINNY L BUMGARDNER · 2023 to 2026
$1.2M
Microfluidics Array Based Sorting, Isolation, and RNA Analysis in Single Extracellular V csiclesUG3TR002884 · NCATS · OHIO STATE UNIVERSITY · PI KIM, BETTY, REATEGUI, EDUARDO · 2019 to 2020
$822k
Machine Learning-enabled Classification of Extracellular Vesicles Using Nanoplasmonic MicrofluidicsR00EB033857 · NIBIB · NORTHWESTERN UNIVERSITY · PI Colin Lee Hisey · 2024 to 2026
$668k
In situ capsid protein and DNA imaging platform for the characterization of therapeutic adeno-associated virus (AAV) titersR21TR005565 · NCATS · OHIO STATE UNIVERSITY · PI REATEGUI, EDUARDO · 2025 to 2025
$374k
Machine Learning-enabled Classification of Extracellular Vesicles Using Nanoplasmonic MicrofluidicsK99EB033857 · NIBIB · OHIO STATE UNIVERSITY · PI HISEY, COLIN LEE · 2023 to 2024
$200k
NCATS NIH HHS R21 TR005565NCATS NIH HHS T32 TR004543NCATS NIH HHS U18 TR003807NCATS NIH HHS UG3 TR002884NCI NIH HHS P01 CA229123NCI NIH HHS P50 CA236733NCI NIH HHS R35 CA197570NCI NIH HHS UH3 CA241685NHLBI NIH HHS T32 HL149637NIBIB NIH HHS K99 EB033857NIBIB NIH HHS R00 EB033857U.S. Department of Health & Human Services | NIH | National Center for Advancing Translational Sciences (NCATS) U18TR003807U.S. Department of Health & Human Services | NIH | National Center for Advancing Translational Sciences (NCATS) UG3/UH3TR002884
6 · The paper itself

Abstract

The role of extracellular vesicles (EVs) and particles (EPs/EVPs) in human health and disease has garnered considerable attention over the past two decades. However, while several types of EVPs are known to interact dynamically with the extracellular matrix and there is great potential value in producing high-fidelity EVP micropatterns, there are currently no label-free, scalable and tunable platform technologies with this capability. We introduce light-induced extracellular vesicle and particle adsorption (LEVA) as a powerful solution to study surface-bound EVPs. The versatility of LEVA is demonstrated using GFP-EV standards, EVs from conventional and bioreactor cultures, DiFi exomeres and Escherichia coli EVs, with the resulting patterns used for single-EV fluorescence imaging, cell migration on migrasome-mimetic trails and bacterial EV-mediated neutrophil swarming. LEVA will rapidly advance our understanding of extracellular matrix protein- and surface-bound EVPs and should encourage researchers from many disciplines to create new biomimetic, immunoengineering and other assays.

Indexed as

Extracellular VesiclesLightAdsorptionCell MovementEscherichia coliExtracellular MatrixHumansNeutrophils

Identifiers

PMID41254365
PMCPMC12695670

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.