Evidence map›Paper›PMID 41254353›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2025

Bioconversion of chicken feather waste: optimizing keratinase production by Pseudomonas aeruginosa PA1045 and Alcaligenes faecalis IHB B 6507, characterization and application as organic fertilizers.

Frank Olakunle Otuyelu, Patricia Folakemi Omojasola, Olusoji Olusegun Adebisi, Jonathan Maiangwa, Ridwan Tope Azeez, Aminat Alolade Ali, Barakat Oluwabukunola Saheed, Muinat Olanike Kazeem

Abstract read
PubMed Publisher
In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Frank Olakunle OtuyeluDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Patricia Folakemi OmojasolaDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Olusoji Olusegun AdebisiSchool of Biosciences, College of Health & Life Sciences, Aston University, Birmingham, UK.
Jonathan MaiangwaDepartment of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria.
Ridwan Tope AzeezDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Aminat Alolade AliDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Barakat Oluwabukunola SaheedDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Muinat Olanike KazeemDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria. kazeem.mo@unilorin.edu.ng.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study optimizes keratinase production from chicken-feather waste by Pseudomonas aeruginosa PA1045 and Alcaligenes faecalis IHB B 6507, characterize and apply their hydrolysates for organic fertilizer application.

methodsKeratinolytic activities of P. aeruginosa PA1045 and A. faecalis IHB B 6507 were assessed on chicken feather media. Factors optimized were temperature, pH, substrate concentration and inoculum size. Chicken-feather hydrolysate (CFH) was characterized via Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), protein and amino acid profiling using Bradford method and High-Performance Liquid Chromatography (HPLC) respectively. Following the application of CFH on maize plant, nitrogen content, phytochemical screening, chlorophyll concentration and plant growth parameters were assessed.

resultKeratinolytic activity from P. aeruginosa peaked (42.48 U/mL) at day 5, while A. faecalis was maximum (40.88 U/mL) at day 7. Optimal keratinase production conditions by P. aeruginosa and A. faecalis were temperature 35 °C, pH 8.0, 1.5% substrate concentration and inoculum size of 2 mL and 3 mL, respectively. SEM analysis confirmed feather degradation and loss of structural integrity in hydrolysates (P. aeruginosa- PaH; A. faecalis- AfH) compared to the organized control, while FTIR analysis revealed distinct peaks indicating compositional differences. The PaH demonstrated higher protein (7.46 mg/100 g) and nitrogen (6.688 ± 1.08%) content. L-Threonine was the most dominant amino acid in both hydrolysates. Significantly, plants treated with PaH and AfH displayed enhanced growth compared to NPK fertilizer and water controls.

conclusionP. aeruginosa PA1045 and A. faecalis IHB B 6507 efficiently hydrolyzed chicken feathers under optimal conditions, yielding hydrolysates rich in essential amino acids, underscoring their multifaceted biotechnological applications in feather waste valorization and potential agricultural use.

Indexed as

Alcaligenes faecalisFeathersFertilizersPeptide HydrolasesPseudomonas aeruginosaAnimalsBacterial ProteinsChickensHydrogen-Ion ConcentrationKeratinsTemperatureZea maysBacterial ProteinsFertilizerskeratinaseKeratinsPeptide HydrolasesFeather hydrolysateFeather hydrolysisKeratinolytic bacteriaMaize plant growth improvementOrganic fertilizer

Identifiers

PMID41254353

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.