ArticleClinical and experimental dental research2025
In Vitro Evidence for Oral Soft Tissue Regenerative Effects of a Polynucleotide-Hyaluronan Formulation.
Article in Clinical and experimental dental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Differential Attachment of Engineered Oral Soft Tissues to Implant Surfaces.Dentistry journal · 2026Article
- In Vitro Evidence for Oral Soft Tissue Regenerative Effects of a Polynucleotide-Hyaluronan Formulation.Clinical and experimental dental research · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
objectivesWe aimed to investigate the effects of a polynucleotide-hyaluronan (PN-HA) formulation on human palatal fibroblasts (HPFs) and oral epithelial cells (OKF6/TERT-2) to assess its potential application in oral soft tissue reconstructive procedures. MATERIAL AND
methodsThe impact of PN-HA on cell viability, migration, and proliferation was assessed using functional assays. Gene expression of wound healing-related growth factors, inflammatory cytokines, and keratinization markers was evaluated by qRT-PCR, while protein expression and kinase signaling were analyzed via immunofluorescence and immunoblotting.
resultsPN-HA preserved cell viability and significantly (p < 0.05) enhanced migration of both HPFs and OKF6/TERT-2 compared to untreated control cells. While it significantly (p < 0.01) increased proliferation in HPFs, no direct proliferative effect was observed in OKF6/TERT-2 monocultures. In HPFs, PN-HA upregulated TGFB1, PDGFB, and FGF2 genes encoding the respective pro-migratory/pro-proliferative growth factors, whereas TGFB3 and EGF were selectively induced in OKF6/TERT-2. PN-HA markedly increased pro-inflammatory cytokine expression and activated p38 MAPK signaling in epithelial cells, alongside ERK1/2 activation and moderate increase in adenosine A2A receptor expression in both cell types. A pronounced upregulation of keratinocyte differentiation markers was observed at both mRNA and protein levels. Notably, in epithelial-fibroblast cocultures, PN-HA significantly (p < 0.01) enhanced epithelial proliferation, likely mediated by increased FGF7 and HGF expression in fibroblasts.
conclusionsPN-HA promotes oral fibroblast migration and proliferation, induces epithelial differentiation, and stimulates epithelial proliferation in coculture with fibroblasts. The observed cell- and context-specific effects highlight the potential of PN-HA to support soft tissue regeneration and keratinization in periodontal reconstructive surgeries.
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