ArticleThe Journal of infectious diseases2026
Detection of Carbapenem-Resistant Bacteria in Skilled Nursing Facility Wastewater.
Article in The Journal of infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Evaluation of Detection Methods for Wastewater Surveillance of Antimicrobial-Resistant Bacteria from Healthcare Facilities.Emerging infectious diseases · 2026Article
- Plasmid-borneOne health (Amsterdam, Netherlands) · 2026Article
- Healthcare wastewater surveillance: methodological considerations for sampling, feasibility, and implementation.Journal of water and health · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
backgroundWastewater surveillance at healthcare facilities for carbapenem-resistant (CR) bacteria harboring carbapenemase genes could result in earlier detection of circulating or emerging antimicrobial resistance (AR) threats. However, knowledge gaps exist regarding the CR bacterial community and carbapenemase genes present in facility wastewater and plumbing, which need to be addressed.
methodsWastewater effluent samples from three skilled nursing facilities (SNFs) (Georgia, USA) were collected weekly over six months. Selective chromogenic media were examined for their performance in screening the samples for targeted CR bacteria. Culture-dependent and culture-independent methods (ie, PCR) were applied to enriched wastewater samples (n = 73) to screen isolates and pooled samples for the most common carbapenemase genes circulating in the United States (blaKPC, blaNDM, blaVIM, blaOXA-48-like, and blaIMP), and method sensitivity was compared. Whole genome sequencing (WGS) was used to identify all AR genes present in isolates.
resultsBacteria (n = 209 isolates) were identified using MALDI-TOF and included >20 different genera. Clinically relevant (Acinetobacter baumannii complex, Enterobacter cloacae complex, Enterococcus faecium, Stenotrophomonas spp.) and environmental/opportunistic (Comamonas spp., Pandoraea spp., Pseudomonas putida group) CR bacteria were identified in each of the three SNFs. In Facility A and C, only blaKPC was detected in 9 (26.5%) of 34 and 14 (11.4%) of 123 recovered isolates, respectively. There were no PCR-positive results in Facility B. WGS confirmed the presence or absence of all genes screened by PCR (100% concordance).
conclusionsThese data provide a proof-of-concept and insights into CR bacteria present in healthcare facility wastewater using culture, PCR, and sequencing methods.
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Registered trials
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