Evidence map›Paper›PMID 41251349›Full record

ArticleJournal of virology2025

Conserved tripartite tail proteins mediate virophage-host interactions through

Ting Chu, Qinran Wang, Chen Hu, Zexian Zhou, Bo Li, Yesheng Yan, Xiang Li, Aohan Wang, Qianglin Fang, Shuling Yan and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ting ChuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.ORCID 0000-0002-9073-6817
Qinran WangCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Chen HuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Zexian ZhouCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Bo LiShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China.
Yesheng YanCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Xiang LiCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Aohan WangSchool of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong, China.
Qianglin FangSchool of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong, China.ORCID 0000-0003-1844-7313
Shuling YanEntwicklungsgenetik und Zellbiologie der Tiere, Philipps-Universität Marburg, Marburg, Germany.
Lanming ChenCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.ORCID 0000-0001-9537-8476
Yongxin YuCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.ORCID 0000-0002-1447-2880
Yongjie WangCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, China.ORCID 0000-0002-7209-585X

Funding

National Natural Science Foundation of China 31570112, 32370151
6 · The paper itself

Abstract

Virophages are small double-stranded DNA viruses that parasitize giant viruses and play crucial roles in microbial ecosystem dynamics. In this study, we investigate three evolutionarily conserved tail proteins in Dishui Lake Virophage 2 (DSLV2) that exhibit significant structural homology to the T4 phage gp37 long tail fiber protein. Our integrated approach combining structural modeling, molecular dynamics simulations, and biochemical characterization reveals that these proteins form a stable heterotrimeric complex with a functional receptor-binding domain. Comprehensive genomic analysis of related virophages within the Aquatic Virophage 1 lineage demonstrates the widespread conservation of this tripartite tail architecture, suggesting its fundamental importance in virophage biology. Phylogenetic evidence indicates that these tail proteins of DSLV2 were evolutionarily related to those of IMPORTANCE: This study significantly advances our understanding of virophage biology by elucidating key molecular mechanisms underlying their host interactions. The discovery of a conserved heterotrimeric tail complex in Dishui Lake Virophage 2, evolutionarily related to tail proteins of

Indexed as

BacteriophagesHost Microbial InteractionsLipopolysaccharidesSynechococcusViral Tail ProteinsMolecular Dynamics SimulationPhylogenyProtein BindingLipopolysaccharidesViral Tail Proteinsalgacyanobacteriaheterotrimerreceptor bindingtail fiber proteinvirophage

Identifiers

PMID41251349
PMCPMC12724248

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.