Evidence map›Paper›PMID 41249930›Full record

ArticleBMC microbiology2025

Multidrug resistant hypervirulent ST307 clone from genomic surveillance of extended spectrum beta-lactamase-producing Klebsiella pneumoniae species complex in East Africa.

Benson R Kidenya, Ivan Sserwadda, Stephen Kanyerezi, Inyasi Lawrence Akaro, Baraka Mkinze, Suhaila O Hashim, Everlyne Isoe, Moses L Joloba, Gerald Mboowa, Jeremiah Seni

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Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Benson R KidenyaDepartment of Biochemistry and Molecular Biology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P. O. Box 1464, Mwanza, Tanzania. benkidenya@gmail.com.
Ivan SserwaddaDepartment of Immunology and Molecular Biology, College of Health Sciences, School of Biomedical Sciences, Makerere University, P. O Box 7072, Kampala, Uganda.
Stephen KanyereziDepartment of Immunology and Molecular Biology, College of Health Sciences, School of Biomedical Sciences, Makerere University, P. O Box 7072, Kampala, Uganda.
Inyasi Lawrence AkaroDepartment of Surgery, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P. O. Box 1464, Mwanza, Tanzania.
Baraka MkinzeDepartment of Surgery, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P. O. Box 1464, Mwanza, Tanzania.
Suhaila O HashimDepartment of Biochemistry and Bioinformatics, School of Pure and Applied Sciences, Pwani University, P.O Box 195-80108, Kilifi, Kenya.
Everlyne IsoeDepartment of Biochemistry and Bioinformatics, School of Pure and Applied Sciences, Pwani University, P.O Box 195-80108, Kilifi, Kenya.
Moses L JolobaDepartment of Immunology and Molecular Biology, College of Health Sciences, School of Biomedical Sciences, Makerere University, P. O Box 7072, Kampala, Uganda.
Gerald MboowaDepartment of Immunology and Molecular Biology, College of Health Sciences, School of Biomedical Sciences, Makerere University, P. O Box 7072, Kampala, Uganda. gmboowa@gmail.com.
Jeremiah SeniDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P. O. Box 1464, Mwanza, Tanzania.

Funding

Makerere University Data Science Research Training to Strengthen Evidence-Based Health Innovation, Intervention and Policy (MakDARTA)U2RTW012116 · FIC · MAKERERE UNIVERSITY COLLEGE OF HEALTH SCIENCES · PI William Checkley, Moses Lutaakome Joloba · 2021 to 2026
$2.0M
Bill and Melinda Gates Foundation (BMGF) and the African Academy of Sciences and partners INV038071FIC NIH HHS U2R TW012116
6 · The paper itself

Abstract

backgroundExtended spectrum beta-lactamase-producing Klebsiella pneumoniae species complex (ESBL-KpSC) presents a significant therapeutic challenge to global public health, making it essential to assess the risks associated with recovered isolates. In this study, we utilized whole-genome sequencing (WGS) to comprehensively analyze ESBL-KpSC isolates from hospitalized orthopedic patients, their caretakers, and the hospital environments at tertiary referral hospitals in Uganda and Tanzania, East Africa.

methodsESBL-KpSC isolates were collected between September 2019 and May 2020. Rectal swabs were obtained from patients shortly after their admission to orthopedic wards for ESBL-KpSC screening. Additional swabs were collected from caretakers, healthcare workers, and the surrounding hospital environments. Confirmed ESBL-KpSC isolates underwent DNA extraction for WGS, and the resulting sequences were analyzed to identify species, sequence type (ST), antimicrobial resistance (AMR) genes, virulence genes, and to calculate antimicrobial resistance and virulence scores.

resultsWe analyzed 24 ESBL-KpSC isolates, 7 (29.2%) from Uganda and 17 (70.8%) from Tanzania. Of these, 14 (58.3%) were identified as Klebsiella pneumoniae, 7 (29.2%) as Klebsiella quasipneumoniae subsp. similipneumoniae, and 3 (12.5%) as Klebsiella variicola subsp. variicola. The 24 ESBL-KpSC genomes were distributed across 19 sequence types (STs), indicating a high diversity of 79.2% (19/24). Among the 19 STs, two genomes were found in each of the following STs: ST17, ST307, ST2478, ST367-2LV, and ST3946-1LV, with the remaining genomes being singletons. Of the 24 ESBL-KpSC genomes, 6 (25.0%) had a virulence score greater than 0, and one isolate was identified as multidrug-resistant hypervirulent K. pneumoniae (MDR-hvKp). The most prevalent ESBL gene was bla

conclusionWe report, for the first time, the presence of the MDR-hvKp ST307 clone from East Africa. One out of four ESBL-KpSC isolates was identified as a virulent strain harboring multiple AMR genes, underscoring the urgent need for routine screening of patients with prolonged hospital stays. Additionally, our findings emphasize the critical importance of robust infection prevention and control measures to mitigate the spread of AMR within hospitals in East Africa.

Indexed as

beta-LactamasesDrug Resistance, Multiple, BacterialKlebsiella InfectionsKlebsiella pneumoniaeAnti-Bacterial AgentsFemaleGenome, BacterialHumansMaleMicrobial Sensitivity TestsTanzaniaUgandaVirulenceVirulence FactorsWhole Genome SequencingAnti-Bacterial Agentsbeta-LactamasesVirulence FactorsCloneEast africaExtended spectrum beta-lactamase-producing Klebsiella pneumoniae species complex (ESBL-KpSC)Genomic surveillanceKlebsiella pneumoniae ST307Multidrug-resistant hypervirulent

Identifiers

PMID41249930
PMCPMC12625365

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.