ArticleScientific reports2025
Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PSmOrange and PSmOrange2 fluorescent proteins undergo irreversible photoconversion from the orange to far-red form under blue light, which makes them probes of choice for protein tracking and single-molecule super-resolution imaging. However, both proteins exhibit noticeable photoconversion under 550-570 nm light used for excitation of their orange form, which complicates applications of these photoconvertible FPs in cell imaging experiments. Here, we report the next-generation PSmOrange variant, called PSmOrange3, which is characterized by minimal photoconversion under 550-570 nm light and high photoconversion contrast. PSmOrange3 undergoes efficient photoconversion from the orange (Ex/Em at 550 nm/564 nm) to far-red form (Ex/Em at 614 nm/655 nm) with 430-470 nm violet-blue light of moderate power density (3-180 mW/mm
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.