Evidence map›Paper›PMID 41249232›Full record

ArticleScientific reports2025

Characterization of the spectrum of insecticidal activity of Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins produced by lepidopteran-protected MON 89151 cotton.

Carlos J Esquivel, Frankie Stubbins, Harit K Bal, Jianguo Tan, Brent Werner, Steven Michael Reyna, Jennifer Fridley, Geoffrey Mueller, Yu Liu-Gontarek, Bingyao Li and 6 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Carlos J EsquivelBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA. carlos.esquivelpalma@bayer.com.
Frankie StubbinsBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Harit K BalBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Jianguo TanBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Brent WernerBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Steven Michael ReynaBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Jennifer FridleyBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Geoffrey MuellerBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Yu Liu-GontarekBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Bingyao LiBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Chen MengBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Christopher R BrownBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Tianbo XuBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Steven L LevineBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Yong YinBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA.
Chitvan KhajuriaBayer CropScience LP, Regulatory Science, 700 Chesterfield Parkway West, Chesterfield, MO, 63017, USA. chitvan.khajuria@bayer.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The spectrum of insecticidal activity of the Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins targeting lepidopteran insect pests produced by MON 89151 cotton was characterized with microbially-produced protein-incorporated diet bioassays against selected insect species from four orders and nine families. Cry1B.3 and Cry1Da_7 are Bacillus thuringiensis (Bt) insecticidal proteins, whereas the Vip3Cb1 protein is derived from Paenibacillus spp. in the P. popilliae-containing clade. Notably, Cry1B.3 has the same functional region as the Cry1B.2 protein in MON 94637 soybean, and Cry1Da_7 is identical to the protein expressed in MON 95379 maize; therefore, existing spectrum of activity data for Cry1B.2 and Cry1Da_7 were utilized to inform the characterization of Cry1B.3 and Cry1Da_7 in MON 89151 cotton. Overall, our results demonstrate that Cry1B.3, Cry1Da_7, and Vip3Cb1 exhibit a narrow spectrum of activity limited to the lepidopteran pests tested. Thus, these insecticidal proteins are valuable additions to the cotton trait portfolio to manage lepidopteran pests with a high degree of specificity.

Indexed as

Bacterial ProteinsEndotoxinsGossypiumHemolysin ProteinsInsecticidesLepidopteraAnimalsBacillus thuringiensisBacillus thuringiensis ToxinsPest Control, BiologicalPlants, Genetically ModifiedBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisInsecticidesActivity spectrumCry proteinInsect-protected cottonMON 89151Vip3Cb1 protein

Identifiers

PMID41249232
PMCPMC12623414

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.