Evidence map›Paper›PMID 41248896›Full record

ArticleClinical and molecular hepatology2026

Novel near-infrared probe for monitoring lipid peroxidation-mediated viscosity change in ferroptotic hepatocytes.

Le Bich Hang Pham, Taeeung Kim, Seoyoung Kim, Yun Seok Kim, Jiyeon Kim, Kyeongseon Kim, Hyeonwoo Lim, Wan Seob Shim, Byoungmo Kim, So-Yeol Yoo and 5 more

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Article in Clinical and molecular hepatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Le Bich Hang PhamResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Taeeung KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Seoyoung KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Yun Seok KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Jiyeon KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Kyeongseon KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Hyeonwoo LimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Wan Seob ShimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Byoungmo KimResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
So-Yeol YooResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Jae-Young LeeResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Murim ChoiDepartment of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
Won KimDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul Metropolitan Government Boramae Medical Center, Seoul, Korea.
Keon Wook KangResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
Jeeyeon LeeResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.

Funding

Ministry of Science, ICT and Future Planning RS-2022-NR067269National Research Foundation of Korea RS-2021-NR059390
6 · The paper itself

Abstract

BACKGROUND/

aimsFerroptosis, recently emerged as a new cell death modality characterized by iron-dependent peroxidation of lipids, has been explored in various diseases. However, detection of ferroptosis, particularly in chronic liver disease models, is hampered by the lack of universal ferroptosis markers and limited number of fluorescence sensors for in vivo ferroptosis.

methodsIn this study, we developed TTM-4 as a highly sensitive near-infrared (NIR) fluorescent probe to detect ferroptosis.

resultsTTM-4 exhibited turn-on fluorescence upon viscosity change, enabling visualization of lipid peroxidation (LPO) in ferroptotic hepatocytes and liver tissue samples with greater sensitivity than BODIPY 581/591 C11. Timelapse live-cell imaging of erastin-treated cells revealed real-time LPO dynamics involving cytosolic lipid droplets (cLDs), endoplasmic reticulum, and nuclear LDs in a chronological order. Further gene expression analysis of 216 liver tissue samples from the NCBI GEO database showed a significant increase in CIDEC concurrent with TTM-4 fluorescence during progression to metabolic dysfunction-associated steatotic hepatitis (MASH). TTM-4, with its low toxicity and turn-on NIR emission during ferroptosis, also enabled in vivo visualization of ferroptosis in liver injury and metabolic dysfunction-associated steatotic liver disease (MASLD) models.

conclusionsOur findings suggest that TTM-4 enables monitoring of ferroptosis in MASLD and would aid in early MASH diagnosis.

Indexed as

FerroptosisFluorescent DyesHepatocytesLipid PeroxidationAnimalsHumansLipid DropletsLiverMaleMiceViscosityFluorescent DyesFerroptosisMetabolic dysfunction-associated steatotic liver diseaseNear-infrared emissive probeNuclear lipid dropletsViscosity

Identifiers

PMID41248896
PMCPMC12835794

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.