Evidence map›Paper›PMID 41248300›Full record

ArticleJournal of agricultural and food chemistry2025

Proteomic Characterization of Major Fish Allergy Responsive Protein Parvalbumins in Hilsa (

Nazma Shaheen, Zongkai Peng, Amit Singh, Asfia Wahab, Maria Gasset, Md Hafizul Islam, Oumma Halima, Aleena F Ali, Salmaan M Shah, Malek Salkini and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of agricultural and food chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Nazma ShaheenInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, Bangladesh.
Zongkai PengDepartment of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, United States.
Amit SinghDepartment of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, United States.
Asfia WahabDepartment of Biology, University of York, York YO10 5DD, U.K.
Maria GassetInstitute of Physical-Chemistry Blas Cabrera,Spanish National Research Council, Madrid 28006, Spain.
Md Hafizul IslamInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, Bangladesh.
Oumma HalimaInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, Bangladesh.
Aleena F AliDodge Family College of Arts and Sciences, University of Oklahoma, Norman, Oklahoma 73019, United States.
Salmaan M ShahDodge Family College of Arts and Sciences, University of Oklahoma, Norman, Oklahoma 73019, United States.
Malek SalkiniDodge Family College of Arts and Sciences, University of Oklahoma, Norman, Oklahoma 73019, United States.
Saaim SaleemiDodge Family College of Arts and Sciences, University of Oklahoma, Norman, Oklahoma 73019, United States.
Abdullah F MallahDodge Family College of Arts and Sciences, University of Oklahoma, Norman, Oklahoma 73019, United States.
Ayan KhanDepartment of Biology, University of Central Oklahoma, Edmond, Oklahoma 73034, United States.
Sohaib MesiyaDepartment of Biology, University of Central Oklahoma, Edmond, Oklahoma 73034, United States.
Morshed KhandakerNanobiology Laboratory, School of Engineering, University of Central Oklahoma, Edmond, Oklahoma 73034, United States.
Aayan ZarifMercy School Institute, Edmond, Oklahoma 73013, United States.
Akbar AliMass Spectrometry, Proteomics and Metabolomics Core Facility, Stephenson Life Sciences Research Center, The University of Oklahoma, Norman, Oklahoma 73019, United States.
Amir SamourMass Spectrometry, Proteomics and Metabolomics Core Facility, Stephenson Life Sciences Research Center, The University of Oklahoma, Norman, Oklahoma 73019, United States.
John W PetersDepartment of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, United States.ORCID 0000-0001-9117-9568
Zhibo YangDepartment of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, United States.ORCID 0000-0003-0370-7450
Nagib AhsanDepartment of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, United States.ORCID 0000-0002-6813-7527

Funding

Novel single-cell mass spectrometry methods to assess the role of intracellular drug concentration and metabolism in antimicrobial treatment failureR01AI177469 · NIAID · UNIVERSITY OF OKLAHOMA · PI Laura-Isobel McCall, Zhibo Yang · 2023 to 2026
$1.6M
Defining the mechanistic determinants of catalytic bias in cofactor-based enzymatic oxidation-reduction reactionsR01GM138592 · NIGMS · WASHINGTON STATE UNIVERSITY · PI PETERS, JOHN W · 2020 to 2023
$1.4M
NIAID NIH HHS R01 AI177469NIGMS NIH HHS R01 GM138592
6 · The paper itself

Abstract

Parvalbumin-beta (PRVB) is the major allergen in bony fish; however, its characteristics in Hilsa, an economically important anadromous fish in Southeast Asia, remain unclear. This study characterized Hilsa PRVs using proteomic approaches across two major riverine habitats and developmental stages. Three PRV isoforms were identified in Hilsa muscle through unique peptide sets. Label-free quantitative proteomics, supported by ELISA, revealed higher PRVB levels in juvenile Hilsa compared with adults, independent of habitat. Comparative analysis showed that Hilsa contained significantly lower PRVB than other freshwater species. Additionally, multiple reaction monitoring (MRM)-based targeted proteomics demonstrated PRV's potential as a marker for allergen quantitation and for authenticating Hilsa in mixed fish samples. These findings provide novel insights into Hilsa PRVs, offering a foundation for future studies of fish quality, allergen assessment, and food safety.

Indexed as

AllergensFish DiseasesFishesFish ProteinsFood HypersensitivityParvalbuminsAnimalsAsia, SoutheasternProteomicsSeafoodAllergensFish ProteinsParvalbuminsallergensClupeidaedevelopmental stagefood safetytargeted proteomics

Identifiers

PMID41248300
PMCPMC12670419

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.