Evidence map›Paper›PMID 41248285›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

High-resolution single-cell analyses reveal evolutionary constraints and evolvability of sexual circuits in

Justin T Walsh, Ian P Junker, Yu-Chieh David Chen, Yen-Chung Chen, Helena Gifford, Dawn S Chen, Yun Ding

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Evolution of compound eye cell types shapes visual behaviors acrossbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Organization and evolution of sex-biased gene expression inbioRxiv : the preprint server for biology · 2026
    Article
  4. Molecular Basis of Behavioral Diversity in a Sibling Species Trio.bioRxiv : the preprint server for biology · 2026
    Article
  5. The neural substrates of behavioral evolution: Divergent behaviors from conserved neurons.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. High-resolution single-cell analyses reveal evolutionary constraints and evolvability of sexual circuits inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Justin T WalshDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19096.ORCID 0000-0001-9989-7089
Ian P JunkerDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19096.
Yu-Chieh David ChenDepartment of Biology, New York University, New York, NY 10003.ORCID 0000-0002-2597-7577
Yen-Chung ChenDepartment of Biology, New York University, New York, NY 10003.ORCID 0000-0002-8529-1251
Helena GiffordDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19096.
Dawn S ChenDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19096.ORCID 0000-0002-0198-2216
Yun DingDepartment of Biology, University of Pennsylvania, Philadelphia, PA 19096.ORCID 0000-0001-8915-8434

Funding

From association to causation: pinpointing the genetic and molecular determinants underlying natural behavioral variationsR35GM142678 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI DING, YUN · 2021 to 2025
$2.2M
HHS | NIH | National Eye Institute (NEI) NIH National Eye InstituteHHS | NIH | National Institute of General Medical Sciences (NIGMS) R35GM142678New York State Stem Cell Science (NYSTEM) #C322560GGNew York University (NYU) MacCracken FellowshipNIGMS NIH HHS R35 GM142678
6 · The paper itself

Abstract

Understanding how the cellular and molecular composition of neural circuits evolves to generate species-specific behaviors remains a major challenge in evolutionary biology and neuroscience. The remarkable diversity of male sexual behaviors among

Indexed as

Biological EvolutionDrosophilaSexual Behavior, AnimalSingle-Cell AnalysisAnimalsDNA-Binding ProteinsDrosophila melanogasterDrosophila ProteinsEvolution, MolecularFemaleMaleNerve Tissue ProteinsNeuronsTranscription FactorsTranscriptomeDNA-Binding ProteinsDrosophila ProteinsDSX protein, Drosophilafru protein, DrosophilaNerve Tissue ProteinsTranscription Factorsbehavioral evolutionevolution of cell typesneural circuit evolutionneuropeptide signalingsingle-cell transcriptomics

Identifiers

PMID41248285
PMCPMC12663948

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.