ArticlePLoS neglected tropical diseases2025
Rapid respiratory cryptococcosis detection using targeted next-generation sequencing.
Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Timing of Whole Lung Lavage in Autoimmune Pulmonary Alveolar Proteinosis with Concurrent Opportunistic Infection: A Case Report and Systematic Review.Infection and drug resistance · 2026Article
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Authors and funding
8 authors.
Funding
Abstract
Cryptococcus neoformans, a WHO critical-priority and neglected fungal pathogen, causes cryptococcosis primarily via inhalation. Clinical manifestations range from asymptomatic infection to fatal meningitis; suboptimal diagnostics cause delayed detection and high meningitis mortality. This retrospective study evaluated the clinical utility of targeted next-generation sequencing (tNGS) for diagnosing pulmonary cryptococcosis using respiratory specimens, compared to serum cryptococcal antigen (CrAg) testing and fungal culture. Over 38 months, 39 patients with confirmed cryptococcosis were enrolled. tNGS detected Cryptococcus neoformans in 92.3% (36/39) of respiratory samples, significantly outperforming fungal culture (23.1%, 9/39; P < 0.05). Serum CrAg testing was positive in 64.7% (22/34) of tested patients. tNGS identified C. neoformans in 12 CrAg negative cases and 75% (9/12) of culture-negative cases, enhancing diagnostic yield. The median turnaround time for tNGS (23 [IQR: 21-43] hours) was substantially shorter than for fungal culture (112 [IQR: 77.5-120.5] hours; Mann-Whitney U test, P < 0.05). Higher tNGS read counts correlated with culture positivity (P < 0.05) but not with CrAg status. Polymicrobial infections were detected in 76.9% (30/39) of tNGS tests, underscoring its utility in comprehensive pathogen identification. tNGS of respiratory specimens demonstrates superior sensitivity and markedly shorter turnaround time than fungal culture for cryptococcosis diagnosis. Crucially, its ability to detect C. neoformans in serum CrAg negative patients enables earlier diagnosis in cases missed by standard serology.
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