ArticleEnvironmental microbiology reports2025
Beyond Methane Oxidation: The Protein Landscape of ANME-2a Reveals an Integrated System for Diazotrophy and Membrane Fortification.
Article in Environmental microbiology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- In silico interactome analysis reveals distinct and complementary metabolic roles of bacteria in stingless bee larval food.Computational and structural biotechnology journal · 2026Article
- Beyond Methane Oxidation: The Protein Landscape of ANME-2a Reveals an Integrated System for Diazotrophy and Membrane Fortification.Environmental microbiology reports · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
While renowned for mitigating methane emissions via anaerobic oxidation of methane (AOM), the full ecological strategy of ANME-2a archaea still requires further exploration. This study looks beyond methane oxidation to map the protein landscape of ANME-2a, revealing an integrated system for metabolic autonomy and environmental resilience in this specific isolate. Using a feature-based protein network derived from 230 Methanosarcinales genomes, we uncovered a sophisticated modular architecture. Key findings demonstrate that the AOM machinery is not isolated but functionally coupled with distinct modules dedicated to auxiliary functions. Our analysis not only confirms that this ANME-2a isolate possesses the complete genomic toolkit for autonomous diazotrophy but also reveals the molecular blueprint for its integration with AOM. We show how key machinery has been specialised to support the organism's core energy metabolism, with its core nitrogenase components co-clustered within the same functional module as AOM electron transfer proteins. Furthermore, we identified a specialised module dedicated to 'membrane fortification' through the significant enrichment of pathways for archaeal lipid biosynthesis. This modular blueprint, which integrates core energy metabolism with nitrogen fixation and structural lipid production, provides a model for how diazotrophic ANME-2a lineages may thrive as robust, self-sufficient organisms adapted to dynamic, resource-limited ecosystems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.