Evidence map›Paper›PMID 41247557›Full record

ArticlePlant cell reports2025

Functional insights into JrWOX5: a WOX transcription factor regulating adventitious rooting and plant architecture in walnut.

Rui-Xue Gao, Pu Zhang, Ying-Ying Chang, Li Song, Xiao-Bo Song, Dong Pei

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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rui-Xue GaoState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Pu ZhangState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Ying-Ying ChangCollege of Life Sciences, He'nan Normal University, Xinxiang, 453007, He'nan, China.
Li SongState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Xiao-Bo SongState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China. xiaobo.song@caf.ac.cn.ORCID http://orcid.org/0000-0001-5259-3387
Dong PeiState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.

Funding

Forestry and Grassland Science and Technology Innovation and Development Project 2025132018National Natural Science Foundation of China 32101479
6 · The paper itself

Abstract

key messageJrWOX5 promotes adventitious root formation and modulates plant architecture by interacting with key developmental regulators, providing novel insights into WOX-mediated organogenesis in woody plants. WUSCHEL-related homeobox (WOX) transcription factors, a plant-specific gene family, play essential roles in regulating plant development, including stem cell maintenance and organogenesis. Among the WOX genes identified in Juglans regia, JrWOX5 exhibited significantly elevated expression during AR formation, suggesting a potential regulatory role in this process. To investigate its function, we employed a combination of bioinformatics analysis, subcellular localization, heterologous overexpression, yeast two-hybrid (Y2H) assays, and bimolecular fluorescence complementation (BiFC). The JrWOX5 protein was localized to the nucleus. Ectopic expression of JrWOX5 in transgenic poplar markedly promoted AR formation and altered plant architecture, characterized by increased lateral branching and reduced plant height. To elucidate the molecular mechanism, protein-protein interaction (PPI) network analysis was conducted, and key candidate interactors were experimentally validated. JrWOX5 was found to interact with JrLBD16, a LOB domain-containing protein; JrLHW, a transcription factor; and JrCNR8, a regulator of cell proliferation. These findings indicate that JrWOX5 interacts with developmental regulators, which may jointly affect organogenesis and plant architectural patterning. This study aims to provide a new insight into WOX-associated organogenesis in woody plants.

Indexed as

Homeodomain ProteinsJuglansPlant ProteinsPlant RootsTranscription FactorsGene Expression Regulation, PlantPlants, Genetically ModifiedPopulusProtein Interaction MapsHomeodomain ProteinsPlant ProteinsTranscription FactorsAdventitious root formationEctopic expressionJrWOX5WalnutYeast two-hybrid assay

Identifiers

PMID41247557

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.