ArticleNicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco2026
Human Primary Mesenchymal Stem Cells Fail to Recover Full Osteogenic Differentiation Potential Following Washout of Electronic Cigarette Aerosol Extracts.
Article in Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionElectronic cigarettes (e-cigarettes) are promoted as safer alternatives to combustible cigarettes. However, the health burden and effects of their long-term use remain unidentified. This study evaluates the extent and reversibility of cellular damage in human bone marrow-derived mesenchymal stem cells (MSCs), following in vitro exposure to cigarette and e-cigarette smoke aerosols.
methodsHuman primary MSCs were seeded and exposed to combustible or e-cigarette smoke aerosol extracts for 2 or 3 weeks. MSCs were then assessed for survival and membrane protein expression and distribution. In addition, osteogenic differentiation potential was examined after addition of a differentiation cocktail made up of dexamethasone, ascorbic acid, and β-glycerophosphate.
resultsMSC-mediated cellular repair, described in terms of MSC proliferation and osteogenic differentiation, was compromised by both cigarette and e-cigarette aerosol extracts. MSCs exhibited partial recovery of their proliferative ability after smoke washout. Their membrane proteins were downregulated, and their distribution was altered on the plasma membrane. MSCs showed signs of early osteogenic differentiation following washout of e-cigarette, but matrix mineralization (indicative of terminal differentiation) was hampered, similarly to combustible cigarette aerosols.
conclusionsThese results provide insights on the safety of e-cigarettes and the potential risks they pose to regenerative health, help explain impaired tissue repair mechanisms in chronic smokers, and can serve to inform future public policies on tobacco control. IMPLICATIONS: This study provides insight on the impact of electronic cigarettes on repair mechanisms mediated by stem cells. Importantly, it also examines the consequences of discontinuing combustible or electronic cigarette smoking on stem cell outcomes, notably proliferation, cell integrity, and differentiation potential. Findings from this work feed into the growing evidence on the harmful and persistent effects of electronic smoking modalities.
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