Evidence map›Paper›PMID 41247244›Full record

ArticleMicrobiology spectrum2026

Tandem split-GFP influenza A viruses for sensitive and accurate replication analyses.

Himadri Nath, Ariel Arndt, Joshua T Mann, Steven F Baker

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Himadri NathInfectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, New Mexico, USA.ORCID 0000-0003-2213-6278
Ariel ArndtInfectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, New Mexico, USA.
Joshua T MannInfectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, New Mexico, USA.
Steven F BakerInfectious Disease Program, Lovelace Biomedical Research Institute, Albuquerque, New Mexico, USA.ORCID 0000-0001-8379-283X

Funding

American Lung Association ERPALA2023
6 · The paper itself

Abstract

Reporter influenza A viruses (IAVs) are valuable tools for studying virus fitness, screening antivirals, and assessing host-virus interactions. However, the compact and segmented nature of the IAV genome presents major challenges for engineering genetically stable reporter viruses with minimal fitness defects. To address this, we developed a replication-competent IAV incorporating a tandem split-GFP reporter strategy. Specifically, we appended seven tandem repeats of the GFP11 peptide (GFP11×7) to the C-terminus of the PB2 or PA polymerase subunit. Viruses either contained or lacked a self-cleaving 2A peptide between the viral and reporter genes, thereby creating polymerase fusion proteins or released GFP, respectively. These viruses are complemented in

Indexed as

Green Fluorescent ProteinsInfluenza A virusVirus ReplicationAnimalsAntiviral AgentsCell LineDogsGenes, ReporterHumansInfluenza, HumanMadin Darby Canine Kidney CellsRNA-Dependent RNA PolymeraseViral ProteinsAntiviral AgentsGreen Fluorescent ProteinsRNA-Dependent RNA PolymeraseViral Proteinshigh-throughput screeninginfluenza virusreporter virus

Identifiers

PMID41247244
PMCPMC12772311

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.