Evidence map›Paper›PMID 41247050›Full record

ArticleMicrobiology spectrum2026

Application of FreezeTB, a targeted nanopore sequencing assay, for identification of drug resistance and lineages among pulmonary tuberculosis cases in Alaska.

Bryce Inman, Jeremy Butler, Soren George-Nichol, Ganna Kovalenko, Theresa Savidge, Yvette Vergnetti, Catherine Pongratz, Elizabeth Bee, Andrew R DiNardo, Alexander Kay and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Bryce Inman *Department of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.
Jeremy Butler *Department of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.
Soren George-NicholDepartment of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.
Ganna KovalenkoDepartment of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.
Theresa SavidgeAlaska TB Program, Division of Public Health, Alaska Department of Health, Anchorage, Alaska, USA.
Yvette VergnettiAlaska TB Program, Division of Public Health, Alaska Department of Health, Anchorage, Alaska, USA.
Catherine PongratzAlaska TB Program, Division of Public Health, Alaska Department of Health, Anchorage, Alaska, USA.
Elizabeth BeeAlaska TB Program, Division of Public Health, Alaska Department of Health, Anchorage, Alaska, USA.
Andrew R DiNardoGlobal Tuberculosis Program, Baylor College of Medicine, Houston, Texas, USA.
Alexander KayGlobal Tuberculosis Program, Baylor College of Medicine, Houston, Texas, USA.
Anna MandalakasGlobal Tuberculosis Program, Baylor College of Medicine, Houston, Texas, USA.
Eric BortzDepartment of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.ORCID 0000-0002-1376-0012
Tara E NessDepartment of Biological Sciences, University of Alaska, Anchorage, Alaska, USA.ORCID 0000-0002-4425-418X

Funding

The Administrative CoreP20GM103395 · NIGMS · UNIVERSITY OF ALASKA FAIRBANKS · PI JASON L BURKHEAD · 2012 to 2026
$66.1M
NIAID Centers of Excellence for Influenza Research and Response: Universal Influenza Vaccine Research Activities75N93021C00014 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GARCIA-SASTRE, ADOLFO · 2021 to 2025
$62.6M
Quantifiable stool-based TB PCR to Improve Diagnostics and Treatment MonitoringR01AI137527 · NIAID · BAYLOR COLLEGE OF MEDICINE · PI MANDALAKAS, ANNA M · 2019 to 2022
$2.7M
Wastewater surveillance to enhance the public health response to HIV and TB in EswatiniDP1HD115427 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI Alexander William Kay · 2024 to 2026
$1.7M
Developing Clinical Prediction Tools to Define Strategies for Differentiated Service Delivery in Children and Adolescents Living with HIV in sub-Saharan Africa.K01TW011482 · FIC · BAYLOR COLLEGE OF MEDICINE · PI KAY, ALEXANDER WILLIAM · 2020 to 2024
$749k
FIC NIH HHS K01 TW011482NIAID NIH HHS 75N93021C00014NIAID NIH HHS R01 AI137527NICHD NIH HHS DP1 HD115427NIGMS IDeA Alaska INBRE P20GM103395NIGMS NIH HHS P20 GM103395NIH HHS 1DP1HD115427NIH HHS 1K01TW011482NIH HHS R01AI137527St. Judes/Pediatric Infectious Disease SocietyThrasher Research Fund 01098Thrasher Research Fund 14323
6 · The paper itself

Abstract

Alaska has the highest incidence of tuberculosis (TB) in the United States, with 8% mortality while undergoing TB treatment. With a quarter of TB cases lacking sputum culture to enable drug resistance testing, FreezeTB aimed to develop tools tailored to meet the challenges in Alaska while being translatable to other settings. We designed a rapid and cost-effective laboratory workflow and software to identify drug-resistant mutations in

Indexed as

Antitubercular AgentsMycobacterium tuberculosisNanopore SequencingTuberculosis, Multidrug-ResistantTuberculosis, PulmonaryAlaskaHigh-Throughput Nucleotide SequencingHumansMicrobial Sensitivity TestsMutationWhole Genome SequencingAntitubercular AgentsAlaskaAMRDR-TBMDR-TBMycobacterium tuberculosisnanoporesequencingspoligotypingtargetedTBtNGStuberculosis

Identifiers

PMID41247050
PMCPMC12772309

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.