Evidence map›Paper›PMID 41246415›Full record

ArticleOpen veterinary journal2025

Age-related serological response to H9N2 infection in southwest Tripoli, Libya.

Imad Benlashehr, Ahmed Shaban Kassem Agha, Khalid Mohammed Naffati, Salah Abdulhadi Bshina, Ahmed Abdulmajed Khashkhosha, Abdulatif A Asheg, Abdulwahab M Kammon

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Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Imad BenlashehrDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Ahmed Shaban Kassem AghaDepartment of Microbiology, Libyan Center for Biotechnology Research, Tripoli, Libya.
Khalid Mohammed NaffatiDepartment of Microbiology, Libyan Center for Biotechnology Research, Tripoli, Libya.
Salah Abdulhadi BshinaDepartment of Medicine, Faculty of Veterinary Medicine, Azzaytuna University, Tarhuna, Libya.
Ahmed Abdulmajed KhashkhoshaDepartment of Microbiology, Libyan Center for Biotechnology Research, Tripoli, Libya.
Abdulatif A AshegDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Abdulwahab M KammonDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Avian influenza virus H9N2 is a low-pathogenic subtype that causes respiratory disease and economic losses in poultry and carries zoonotic potential. The virus is endemic across Asia, the Middle East, and North Africa, often exacerbated by co-infections with other pathogens. In Libya, H9N2 was first detected in 2006, but data on age-related immune responses in broilers remain limited. Aim: This study investigated the age-related serological response to H9N2 in southwest Libyan broiler flocks to provide baseline data for vaccination and control programs. Methods: A total of 342 blood samples were collected from six unvaccinated broiler flocks. Antibodies against influenza A were detected by indirect enzyme-linked immunosorbent assay, and H9N2-specific antibodies were measured by hemagglutination inhibition assay. Results: The seropositivity for influenza A increased with age (30%, 52%, and 100% at 10, 24, and 38 days, respectively). The positivity rates for H9N2 were 0%, 22%, and 72%. The progressive increase confirms the active field circulation and endemicity of H9N2. The absence of detectable antibodies at 10 days indicates strong maternal antibody interference. Conclusion: H9N2 is endemic in Libyan broiler flocks, with continuous exposure reflecting age-dependent seroconversion. Vaccination after the age of 10 days may optimize immune protection by reducing maternal antibody interference. Continuous surveillance and molecular monitoring are essential for guiding effective national control measures and assessing zoonotic risks.

Indexed as

ChickensInfluenza A Virus, H9N2 SubtypeInfluenza in BirdsPoultry DiseasesAge FactorsAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayFemaleHemagglutination Inhibition TestsLibyaSeroepidemiologic StudiesAntibodies, ViralELISAH9N2HILibyaSeroprevalence

Identifiers

PMID41246415
PMCPMC12613227

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.