ArticleACS omega2025
A LRELHLNNN-Gal‑3 Modified Adipose Decellularized Scaffold To Construct Colorectal Cancer Model for Anticancer Drug Screening and Immunotherapy.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
With the rapid advancement of cancer immunotherapy, there is an urgent need for 3D tumor models that authentically replicate the tumor immune microenvironment (TIME) for drug screening. In this study, we engineered an adipose-derived decellularized extracellular matrix (adECM) scaffold functionalized with a novel bifunctional fusion protein, LRELHLNNN-Gal-3, comprising a collagen-binding peptide (LRELHLNNN) and galectin-3 (Gal-3). This design enables stable anchoring of Gal-3 to type I collagen within the scaffold, enhancing tumor cell adhesion and proliferation. Compared to unmodified scaffolds, the LRELHLNNN-Gal-3-modified adECM significantly promoted the proliferation of colorectal cancer cells (MC38, LoVo, and primary ascites-derived tumor cells), with peak proliferation increasing approximately 6-fold by day 7. In chemoresistance assays, tumor cells in the modified scaffold exhibited significantly higher survival rates after 48 h of treatment with 5-FU (77.7% vs 53.0%), oxaliplatin (95.2% vs 67.1%), and capecitabine (69.7% vs 44.8%) compared to unmodified scaffolds and outperformed 2D cultures. Importantly, the modified scaffold also enhanced the survival of CD45
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