Evidence map›Paper›PMID 41244326›Full record

ArticleBioengineering & translational medicine2025

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands.

Eduardo Barbieri, Gina N Mollica, Sobhana A Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs and 5 more

Abstract read
In one paragraph

Article in Bioengineering & translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Eduardo BarbieriDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Gina N MollicaDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Sobhana A SripadaDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Shrirarjun ShastryDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Yuxuan WuDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Arianna MinzoniDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Will SmithDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.
Elena WuestenhagenMerck Life Sciences, KGaA Darmstadt Germany.
Annika AldingerMerck Life Sciences, KGaA Darmstadt Germany.
Heiner GraalfsMerck Life Sciences, KGaA Darmstadt Germany.
Michael S CrapanzanoLigaTrap Technologies LLC Raleigh North Carolina USA.
Oliver RammoMerck Life Sciences, KGaA Darmstadt Germany.
Michael M SchulteMerck Life Sciences, KGaA Darmstadt Germany.
Michael A DanieleJoint Department of Biomedical Engineering North Carolina State University and University of North Carolina at Chapel Hill Raleigh North Carolina USA.
Stefano MenegattiDepartment of Chemical and Biomolecular Engineering North Carolina State University Raleigh North Carolina USA.ORCID https://orcid.org/0000-0001-5633-434X

Funding

FDA HHS R01 FD007481
6 · The paper itself

Abstract

Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for LVVs pseudo-typed with Vesicular Stomatitis Virus G (VSV-G) protein. Camelid antibody ligands were initially discovered and utilized to functionalize a resin with a capacity of 10

Indexed as

affinity chromatographylentivirusmembrane chromatographypeptide ligandsproduct polishing

Identifiers

PMID41244326
PMCPMC12617543

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.