ArticleTropical life sciences research2025
Establishment of High-Throughput Screening Assay using 384-Well Plate for Identification of Potent Antioxidants from Malaysian Local Plants Repository and Phytochemical Profile of
Article in Tropical life sciences research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The increasing prevalence of chronic diseases and oxidative stress-related conditions has led to a growing interest in natural products with potent antioxidant properties. High-throughput screening (HTS) assays offer a promising solution for the rapid evaluation of numerous samples. This study aims to establish a robust HTS assay using the 2-Diphenyl-1-Picrylhydrazyl (DPPH) method to identify natural products with significant antioxidant capabilities, ensuring their safety profiles through cytotoxicity screening and phytochemical profiling. An automated liquid handler with the usage of a 384-well assay plate was employed in optimising and validating the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-HTS assay by calculating the metrics. The HTS metrics include Z-prime (Z') values of 0.72 and 0.63, signal to background (S/B) ratios of 3.54 and 9.02, and coefficient of variation percentages (%C/V) of 4.25 and 6.49 for each primary and secondary screenings, respectively. These values indicated that the HTS assay was excellent. By using optimised HTS-DPPH assay programme, a total of 363 plant extracts were screened and 58 (16%) were found to have potent antioxidant activity (a 'yes' score and EC
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