ArticleAsian journal of transfusion science
Comparative study of the prevalence of HIV using the third- and fourth-generation HIV ELISA in blood donors in South India.
Article in Asian journal of transfusion science. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDifferent generations of ELISA have evolved over the years. With the introduction of the fourth-generation ELISA, there is a claim of higher sensitivity over the third-generation HIV ELISA kits. However, few literatures suggest false positive issues. The present study aimed to assess the seroprevalence of HIV and to see the level of agreement between the third- and fourth-generation ELISA for HIV detection. METHODOLOGY: Prospective cross-sectional study conducted in a tertiary care hospital from November 2021 to May 2023. All positive samples by either the third- or fourth-generation ELISA were included in the study. If any sample was positive in one generation, and the same whole batch was repeated in another generation ELISA. All positive samples were subjected to molecular testing by polymerase chain reaction. Cohen's kappa coefficient was used to see the level of agreement between the thrid and fourth-generation HIV ELISA.
resultsSeventeen samples were positive by the third-generation ELISA versus 50 samples were positive by the fourth-generation ELISA. The prevalence of HIV was found to be 0.9% by the fourth-generation ELISA and 0.3% by the third-generation ELISA. There was a fair level of agreement between the third- and fourth-generation HIV ELISA. Both generations have a sensitivity of 100%, whereas specificity with the third generation was 91% and the fourth was 24%.
conclusionBoth the fourth- and third-generation ELISA are highly sensitive; however, the specificity of the fourth-generation kit is less, leading to more false positive results.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.