ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2026
Clinical evaluation of the DermatoPlex qPCR assay for the detection of dermatophyte fungi and Candida spp.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
purposeDermatomycoses represent the most frequent form of human fungal infection globally. The conventional diagnostic approach to identify dermatomycoses is based on direct microscopic examination (DME) and fungal culture. However, these procedures suffer from a number of significant limitations. We aim to evaluate the clinical performance of the novel DermatoPlex qPCR assay (SpeeDx) in comparison to conventional methods for the detection of dermatophyte fungi and Candida spp. in skin and nail material.
methodsBetween February and July 2024, 1320 samples from 1252 patients were collected for the investigation of dermatophyte fungi at the Navarra University Hospital in Spain. DME, culture and DNA extraction were systematically executed in all specimens, and the qPCR assay was retrospectively tested. The Novaplex™ Dermatophyte qPCR assay (Seegene) was used in case of discrepancies.
resultsThe DermatoPlex qPCR assay performed well for the molecular detection of dermatophyte fungi and Candida spp. in skin and nail material; with an overall agreement of 75.4% when compared with culture. Among discrepancies, the majority (75.6%) were ultimately confirmed as true-positives, while only 12.4% resulted in false-negative results. Overall, sensitivity of the qPCR method for the diagnosis of dermatomycoses was 2.3x and 1.7x greater (1.7x and 1.6x for dermatophytosis) when compared with DME and culture, respectively.
conclusionThis is the largest clinical evaluation of a qPCR method for the diagnosis of dermatomycoses. Considering the greater sensitivity of molecular techniques over conventional procedures, the DermatoPlex assay emerges as a reliable and promising qPCR method for the diagnosis of cutaneous mycoses.
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