Evidence map›Paper›PMID 41240231›Full record

ArticleMolecular biology reports2025

Th2 cell-derived exosomes alleviate central nervous system inflammation and demyelination in experimental autoimmune encephalomyelitis by regulating inflammasomes to inhibit microglia activation.

Xianmin Cao, Yufen Peng, Shimin Liu, Chao Zhou, Liang Gao, Jiajun Xiong, Meihong Zhou, Mengqi Xia, Yingqiong Xiong

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xianmin CaoGraduate School, Jiangxi Medical College of Nanchang University, No.461, Bayi Avenue, Nanchang City, Jiangxi Province, China.
Yufen PengDepartment of Neurology, The First Affiliated Hospital of Nanchang University, No.17 Yongwai Zheng Street, Nanchang City, Jiangxi Province, China.
Shimin LiuDepartment of Neurology, Jiangxi Provincial People's Hospital, No. 152, Aiguo Road, Nanchang City, Jiangxi Province, China.
Chao ZhouDepartment of Neurology, Jiangxi Provincial People's Hospital, No. 152, Aiguo Road, Nanchang City, Jiangxi Province, China.
Liang GaoDepartment of Neurology, The First Affiliated Hospital of Nanchang University, No.17 Yongwai Zheng Street, Nanchang City, Jiangxi Province, China.
Jiajun XiongDepartment of Neurology, The First Affiliated Hospital of Nanchang University, No.17 Yongwai Zheng Street, Nanchang City, Jiangxi Province, China.
Meihong ZhouDepartment of Neurology, The First Affiliated Hospital of Nanchang University, No.17 Yongwai Zheng Street, Nanchang City, Jiangxi Province, China.
Mengqi XiaGraduate School, Jiangxi Medical College of Nanchang University, No.461, Bayi Avenue, Nanchang City, Jiangxi Province, China.
Yingqiong XiongDepartment of Neurology, The First Affiliated Hospital of Nanchang University, No.17 Yongwai Zheng Street, Nanchang City, Jiangxi Province, China. ndyfy06816@ncu.edu.cn.

Funding

the National Natural Science Foundation of China 82160245
6 · The paper itself

Abstract

backgroundMultiple sclerosis (MS) is a chronic autoimmune disease that affects the central nervous system. The prevalence of MS is increasing annually, but its etiology and pathogenesis remain unclear. Exosomes which carry various bioactive substances from parent cells, serve as carriers of immunoregulatory factors. Exosomes derived from MSCs and Tregs have been shown to alleviate clinical symptoms in the experimental autoimmune encephalomyelitis (EAE) model. Therefore, we tried to evaluate the functions of Th2 cell-derived exosomes in EAE model and explore the mechanisms. METHODS AND

resultsFemale C57BL/6 mice were randomly divided into control group, EAE group and EAE + Th2-exos group. Mice in EAE + Th2-exos group were injected with Th2-exosomes on day 16 after EAE induction, and samples were collected 48 h later. Compared to EAE group, Th2-exosome treatment significantly reduced the neurological behavior scores and alleviated inflammatory infiltration and vacuolation in the spinal cord. Exosomes downregulated the ratio of M1 microglia, Th1 and Th17 cells, while the ratio of M2 microglia and Th2 cells was upregulated. Additionally, serum levels of pro-inflammatory cytokines (including IL-1β, IL-6, and TNF-α, primarily secreted by microglia) were significantly reduced, and the expression levels of proteins related to the NLRP3 inflammasome and NF-κB signaling pathway (P-P65, Caspase-1 p20, Cleaved-IL-1β, and NLRP3) decreased notably. The P-P65/P65 ratio was significantly lower.

conclusionTh2-exosomes may alleviate MS clinical symptoms by inhibiting microglial activation through the suppression of the NLRP3 inflammasome and NF-κB signaling pathways, thereby reducing central nervous system inflammation.

Indexed as

Demyelinating DiseasesEncephalomyelitis, Autoimmune, ExperimentalExosomesInflammasomesMicrogliaTh2 CellsAnimalsCentral Nervous SystemCytokinesDisease Models, AnimalFemaleInflammationMiceMice, Inbred C57BLMultiple SclerosisNF-kappa BCytokinesInflammasomesNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseExosomesExperimental autoimmune encephalom-yelitisInflammasomeMicrogliaMultiple sclerosisTh2 cell-derived exosomes

Identifiers

PMID41240231

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.