Evidence map›Paper›PMID 41240194›Full record

ArticleMolecular biology reports2025

Transcriptomic insights into ferroptosis and potential therapeutic targets in central Post-Stroke pain following thalamic hemorrhage.

Yinggang Xiao, Ju Gao, Xiaojin Wei, Yang Zhang, Yali Ge, Cunjin Wang, Tianfeng Huang, Longfei Ma

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Yinggang Xiao *Department of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.
Ju Gao *Department of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.
Xiaojin WeiDepartment of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Yang ZhangDepartment of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.
Yali GeDepartment of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.
Cunjin WangDepartment of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.
Tianfeng HuangDepartment of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China. 18051063400@yzu.edu.cn.
Longfei MaDepartment of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, China. Longfei_ma@zju.edu.cn.

Funding

Class A Medical Technology Key Talent of Northern Jiangsu People's Hospital JSGG13Doctoral Startup Fund of Northern Jiangsu People's Hospital BSQDJ0237Jiangsu Province Traditional Chinese Medicine Science and Technology Development Plan - Young Talent Program QN202423National Natural Science Foundation of China 82172190Research Grant of Northern Jiangsu People's Hospital YJJ230056Yangzhou Natural Science Foundation YZ2024159Yangzhou Talent Program Project YCJH00021
6 · The paper itself

Abstract

backgroundCentral post-stroke pain (CPSP), affecting nearly one-third of stroke survivors with thalamic lesions, is increasingly linked to ferroptosis due to its established role in neuroinflammatory and neurodegenerative pathologies. This study investigates the molecular mechanisms underlying CPSP induced by thalamic hemorrhage, with a focus on the role of ferroptosis.

methodsUtilizing single-nucleus RNA sequencing and bulk RNA sequencing data from a mouse model of thalamic hemorrhage, we conducted a comprehensive analysis to identify key genes associated with CPSP and ferroptosis. We employed various bioinformatics tools, including gene set enrichment analysis (GSEA), genome-wide association study (GWAS), and Connectivity Map (CMAP) for drug prediction. To validate the key genes and ferroptosis markers identified through bioinformatic prioritization, a CPSP mouse model was established via thalamic collagenase IV injection and assessed using pain behavioral tests, RT-qPCR, and ELISA.

resultsIntegrated bioinformatics and experimental analyses identified astrocytic Gja1 as the key contributor to CPSP pathogenesis, showing significant positive correlations with pain severity scores, ferroptosis and immune infiltration levels in thalamic tissues. GWAS analysis confirmed the pathogenic regions of these genes. Additionally, we identified potential therapeutic drugs for CPSP through CMAP analysis.

conclusionOur study suggests that astrocytic Gja1 may represent a potential therapeutic target for CPSP, and implicates ferroptosis as a contributing mechanism. These findings provide a preliminary experimental basis for further investigation into targeted interventions and future therapeutic development.

Indexed as

FerroptosisPainStrokeThalamusTranscriptomeAnimalsCerebral HemorrhageComputational BiologyDisease Models, AnimalGene Expression ProfilingGenome-Wide Association StudyMaleMiceMice, Inbred C57BLCPSPFerroptosisGja1SnRNA-seqThalamic hemorrhage

Identifiers

PMID41240194

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