Evidence map›Paper›PMID 41240148›Full record

ArticleWorld journal of microbiology & biotechnology2025

Isolation of DNA aptamers by Tripartite-hybrid-high throughput SELEX (Tripartite-hybrid-HT SELEX) for the detection of pathogenic Leptospira.

Tzi Shien Yeoh, Hong-Leong Cheah, Ling Fong Yoke, Qasim Ayub, Marimuthu Citartan

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tzi Shien YeohAdvanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia.
Hong-Leong CheahMonash University Malaysia Genomics Platform, School of Science, Monash University Malaysia, Bandar Sunway, 47500, Darul Ehsan, Selangor, Malaysia.
Ling Fong YokeMonash University Malaysia Genomics Platform, School of Science, Monash University Malaysia, Bandar Sunway, 47500, Darul Ehsan, Selangor, Malaysia.
Qasim AyubMonash University Malaysia Genomics Platform, School of Science, Monash University Malaysia, Bandar Sunway, 47500, Darul Ehsan, Selangor, Malaysia.
Marimuthu CitartanAdvanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia. citartan@usm.my.

Funding

PRGS PRGS/1/2023/STG02/USM/02/3
6 · The paper itself

Abstract

Leptospirosis is a zoonosis caused by the pathogenic Leptospira, having an estimated global epidemiology of 1.03 million cases. Early diagnosis of leptospirosis is crucial to prevent its progression into more severe form known as Weil's syndrome. A pragmatic approach for early diagnosis of leptospirosis necessitates the direct detection pathogenic Leptospira itself by a specific molecular recognition element. To achieve this, we have isolated two aptamers against the target LipL32 protein of the pathogenic Leptospira, LepDapt-9149 and LepDapt-5561 by a modified SELEX strategy known as the Tripartite-hybrid-HT SELEX. These two aptamers have K

Indexed as

Aptamers, NucleotideLeptospiraLeptospirosisSELEX Aptamer TechniqueBacterial Outer Membrane ProteinsHumansLipoproteinsAptamers, NucleotideBacterial Outer Membrane ProteinsLipL32 protein, LeptospiraLipoproteinsPathogenic LeptospiraSandwich ELASATripartite-hybrid-HT SELEX

Identifiers

PMID41240148

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.