ArticleBMC plant biology2025
Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Plant virus-based vectors can deliver foreign nucleic acid sequences into host plants for expression, serving as powerful biotechnological tools in agricultural applications, such as plant functional genomic study, agronomic trait improvement, pest management, genome editing. Viruses in the genus Potyvirus represent the largest group of plant-infecting RNA viruses, employing polyprotein processing as the genome expression strategy. A variety of potyvirus-based vectors have been documented, whereas the ones with a simple cloning strategy for simultaneous expression of two heterologous proteins await to be exploited. In this study, we developed an infectious cDNA clone of a potato virus Y (PVY) isolate PVY-N, termed pPVY. Next, we engineered Golden Gate-compatible derivatives of pPVY and pPVMV (an infectious cDNA clone of an attenuated strain of pepper veinal mottle virus) by incorporating Type IIS restriction enzyme sites into the P1/HCPro and NIb/CP junctions of the viral genomes. The resulting recombinant clones, pPVY-2×GoldG and pPVMV-2×GoldG, are highly infectious in their natural Solanaceae hosts. Both vectors allow simultaneous insertion of two reporter genes (mCherry and GFP) for robust expression in Nicotiana benthamiana and N. tabacum (for PVY) and N. benthamiana and pepper plants (for PVMV). Serial passage revealed that the recombinant viruses harboring two heterologous genes were genetically stable. Considered the fact that both viruses have a wide host range in Solanaceae plants, the PVY- and PVMV-based vectors would serve as valuable biotechnological tools and have a promising application prospect.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.