ArticleScientific reports2025
A human relevant in vitro alveolar epithelial barrier model to assess inhaled pollutant hazard.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Relevant in vitro models could reduce the requirement for in vivo testing and allow higher throughput for imperative toxicological or pharmaceutical hazard testing. Models of the alveolar barrier are invaluable when assessing the toxicity of inhaled xenobiotics, though there is a requirement that these models are well-characterised and accurately resemble the relevant human cellular architecture. Here, a triple cell co-culture has been developed using hAELVi, NCI-H441 and differentiated THP-1 cells as models of type 1 and type 2 pneumocytes, and alveolar macrophages, respectively. Through pre-staining each cell type, confocal microscopy was first used to determine cell seeding ratios to hAELVi and NCI-H441 required to achieve a human-relevant 16.44:1 ± 3.29 ratio at the time of air-liquid interface exposure. CellTrackers were then used to ensure that the density of differentiated THP-1 cells was in line with previously published anatomical research at 1 cell/18 × 10
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