Evidence map›Paper›PMID 41238732›Full record

ArticleCommunications biology2025

Parameter optimization for MINFLUX microscopy enabled single particle tracking.

Bela T L Vogler, Giovanni De Angelis, Ziliang Zhao, Christian Eggeling, Francesco Reina

Abstract read
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bela T L VoglerInstitute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany. bela.vogler@uni-jena.de.ORCID http://orcid.org/0000-0002-5598-5738
Giovanni De AngelisInstitute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany.
Ziliang ZhaoInstitute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany.
Christian EggelingInstitute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany. christian.eggeling@uni-jena.de.ORCID http://orcid.org/0000-0002-3698-5599
Francesco ReinaInstitute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany.ORCID http://orcid.org/0000-0001-6752-9089

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MINFLUX fluorescence microscopy is a recently introduced super-resolution approach for studying cellular structures and their dynamics with highest detail. MINFLUX (MFX) performs Single Particle Tracking (SPT) at runtime. The ad hoc signal interpretation necessary to sustain the method relies on several parameters, which need to be optimized in relation to the sample under study, such as fluorescent lipid analogs in membranes, to ensure the fidelity of the measurement. We propose a parameter optimization strategy, give an overview of the most important parameters, present a theoretical upper limit for trackable diffusion rates, and demonstrate MFX-enabled SPT of fast

Indexed as

Single Molecule ImagingDiffusionMicroscopy, Fluorescence

Identifiers

PMID41238732
PMCPMC12618476

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.