Evidence map›Paper›PMID 41238654›Full record

ArticleScientific reports2025

Selectively Inhibition of MAPK pathway showed positive effects on Porcine embryo-derived stem cells pluripotency.

Zhuoran Yu, Mengjiao Zhang, Yanjiao Lv, Minli Liu, Yue Yu, Sirong Li, Sihan Zhou, Chaoqian Jiang, Zicheng Qi, Hongxing Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Cells · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhuoran YuNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Mengjiao ZhangNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Yanjiao LvNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Minli LiuNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Yue YuNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Sirong LiNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Sihan ZhouNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Chaoqian JiangNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Zicheng QiNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Hongxing WangNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Jun SongNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Xiaogang WengNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Xiuqin YangNortheast Agricultural University, Harbin, Heilongjiang Province, China.
Tingsheng YanNortheast Agricultural University, Harbin, Heilongjiang Province, China. yantingsheng@neau.edu.cn.
Zhonghua LiuNortheast Agricultural University, Harbin, Heilongjiang Province, China. liuzhonghua@neau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expanded pluripotent stem cells (EPSCs), derived from pre-implantation embryos in specific culture conditions, can contribute to both embryonic and extraembryonic lineages. Human and mouse EPSCs have been successfully established in the LCDM condition, however, porcine embryo-derived stem cells derived in the LCDM system lack expanded pluripotency. To investigate the species-specific molecular mechanism of porcine embryo-derived stem cells (pLCDM) in LCDM condition, we compared the biological characteristics of pLCDM and porcine expanded potency stem cells (pEPSCs). The results revealed that pLCDM exhibited unique pluripotent traits and limited embryonic lineage differentiation potential, with a strong inclination towards extra-embryonic lineage differentiation. Subsequent investigation showed that (S)-(+) -dimethindene maleate (DIM) could completely inhibit the MAPK signaling pathway in pLCDM, and there were significant differences in the activity of the P38, JNK and ERK branches of the MAPK signaling pathway between pLCDM and pEPSC. Screening key molecular target inhibitors in three branches identified the B-RAF inhibitor SB590885 (SB59) as enhancing porcine embryonic stem cell pluripotency and facilitating lineage differentiation. Further analysis revealed a significant correlation between the expression level of phosphorylated ERK (p-ERK) and the developmental potential of porcine embryo-derived stem cells. These findings contribute valuable insights into the understanding of regulatory mechanism of porcine pluripotency.

Indexed as

Embryonic Stem CellsMAP Kinase Signaling SystemPluripotent Stem CellsAnimalsCell DifferentiationCell LineageCells, CulturedProtein Kinase InhibitorsSwineProtein Kinase InhibitorsERKExpanded pluripotent stem cellsLCDMMAPK signaling pathwayPig

Identifiers

PMID41238654
PMCPMC12618612

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.