ArticleScientific reports2025
Impact of antibiotic-induced gut microbiota modulation on morphine analgesia, tolerance, withdrawal, and neurophysiological changes in mice.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Morphine-induced dysbiosis and metabolic remodeling of the gut microbiota is transmissible via fecal microbiota transplantation in mice.American journal of clinical and experimental immunology · 2026Article
- Classification of intestinal inflammation driven by gut microbiota metabolites: a new paradigm for precision treatment of cardiovascular diseases.Frontiers in microbiology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Morphine, a potent opioid analgesic, is limited in clinical use due to tolerance, dependence, and withdrawal. Recent research highlights the gut microbiota as a crucial regulator of opioid effects through the microbiota-gut-brain axis. This study investigates the impact of antibiotic-induced suppression of the gut microbiota on morphine's analgesic efficacy, tolerance, withdrawal, and related behavioral and neurophysiological changes in male mice. Mice received an 11-day antibiotic regimen followed by acute or repeated morphine administration. Behavioral assays (hot plate, tail flick, naloxone-induced withdrawal) evaluated analgesia, tolerance, and withdrawal, while anxiety-like behaviors were assessed via the elevated plus maze. Fecal samples were analyzed for colony-forming units, and EEG recordings measured brain activity. Antibiotics alone induced analgesia and prevented morphine tolerance in repeated dosing (P < 0.001). Antibiotics also reduced withdrawal severity in chronic morphine-treated mice (P < 0.05) but had no effect after acute morphine administration. Anxiety-like behaviors increased with antibiotics (P < 0.001), and both acute (P < 0.01) and chronic morphine (P < 0.05), without interaction. Antibiotic treatment reduced fecal colony-forming units, most significantly with repeated morphine (P < 0.001). Repeated morphine enhanced brain excitability, which was reduced by antibiotic pre-treatment. These findings suggest that modulating the gut microbiota may provide new strategies for managing opioid dependence and related disorders.
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Registered trials
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