Evidence map›Paper›PMID 41237783›Full record

ArticleStem cell reports2025

Gene expression profiling reveals enhanced nutrient and drug metabolism and maturation of hiPSC-derived intestine-on-chip relative to organoids and Transwells.

Renée Moerkens, Joram Mooiweer, Eline Smits, Marijn Berg, Aarón D Ramírez-Sánchez, Rutger Modderman, Jens Puschhof, Cayetano Pleguezuelos-Manzano, Robert J Barrett, Cisca Wijmenga and 2 more

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Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Renée MoerkensDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Joram MooiweerDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Eline SmitsDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Marijn BergDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Aarón D Ramírez-SánchezDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Rutger ModdermanDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Jens PuschhofEpithelium Microbiome Interaction Laboratory, Microbiome and Cancer Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Cayetano Pleguezuelos-ManzanoHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands.
Robert J BarrettBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; F. Widjaja Foundation Inflammatory Bowel Disease Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Cisca WijmengaDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Iris H JonkersDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands.
Sebo WithoffDepartment of Genetics, University of Groningen, University Medical Center Groningen, 9700 RB Groningen, the Netherlands. Electronic address: s.withoff@umcg.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The human intestinal epithelial barrier is shaped by biological and biomechanical cues, including growth factor gradients and fluid flow. While these factors are known to affect adult stem cell (ASC)-derived intestinal epithelial cells in vitro, their impact on human induced pluripotent stem cell (hiPSC)-derived cells is largely unexplored. Here, we compare the cellular composition and gene expression profiles of hiPSC-derived intestinal epithelial cells exposed to various medium compositions and cultured as organoids, in Transwell and microfluidic intestine-on-chip systems. Modulating key signaling pathways (WNT, NOTCH, bone morphogenetic protein [BMP], and mitogen-activated protein kinase [MAPK]) influenced the presence of dividing, absorptive, and secretory epithelial lineages. Upon differentiation, intestinal epithelial cells expressed genes encoding digestive enzymes, nutrient transporters, and drug-metabolizing enzymes. Notably, these pathways were most enhanced in the intestine-on-chip system, along with an expression profile that suggests a more mature state. These findings highlight the potential of hiPSC-derived intestinal cells to model important intestinal functions and guide the selection of optimal culture conditions for specific applications.

Indexed as

Gene Expression ProfilingInduced Pluripotent Stem CellsIntestinal MucosaIntestinesNutrientsOrganoidsCell Culture TechniquesCell DifferentiationEpithelial CellsHumansLab-On-A-Chip DevicesPharmaceutical PreparationsSignal TransductionTranscriptomeNutrientsPharmaceutical Preparationsdifferentiation mediumgut-on-chiphumaninduced pluripotent stem cellintestinal epithelial barrierintestine-on-chiporganoidorgan-on-chipsmall intestineTranswell

Identifiers

PMID41237783
PMCPMC12744852

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.