ArticlePediatric surgery international2025
Severe inflammation at the anastomotic site is associated with mitochondrial dysfunction following colocystoplasty in rats.
Article in Pediatric surgery international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
purposeIn a rat model of colocystoplasty, we aimed to characterize mitochondrial morphological and functional alterations in neobladder tissue, with a focus on the anastomotic bladder region where prolonged severe inflammation may occur.
methodsMale Lewis rats (8-10 weeks old) underwent colocystoplasty, with neobladder tissues sampled within 5 mm of the anastomosis excluding the colon (A-part) or from the distal bladder (B-part) at 2 and 12 weeks. Sham-operated rats (bladder incision and closure) and intact controls were included (n = 5 each). Analyses included quantitative real-time polymerase chain reaction, hematoxylin and eosin staining, immunofluorescence, transmission electron microscopy, and MitoSOX assays to evaluate inflammation, mitochondrial morphology and function, and oxidative stress.
resultsCompared to the B-part, sham, and control, the A-part exhibited urothelial hyperplasia, marked inflammatory cell infiltration, and upregulated interleukin (IL)-1β and IL-6. Mitochondria in the A-part were swollen and contained inclusion bodies, accompanied by upregulated growth differentiation factor 15 and downregulated heat shock protein 60, indicating impaired viability. Superoxide production was increased, while mitochondrial transcription factor A and peroxisome proliferator-activated receptor gamma coactivator 1-α were less activated in the A-part.
conclusionThese findings demonstrate that colocystoplasty induces persistent oxidative stress and mitochondrial dysfunction at the anastomotic site, associated with chronic inflammation.
Indexed as
Identifiers
41236664What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.