Evidence map›Paper›PMID 41236546›Full record

ArticleArchives of microbiology2025

Metabolomic and systems biology insights into lead stress tolerance of Pennisetum purpureum bioaugmented with Enterobacter cloacae.

Anamika Das, S Sajitha Lulu, Jeevanandam Vaishnavi, Manas Manam, Kanagavel Deepankumar, Jastin Samuel, Jabez W Osborne

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Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Anamika DasSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
S Sajitha LuluSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Jeevanandam VaishnaviSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Manas ManamSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Kanagavel DeepankumarSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Jastin SamuelSchool of Biosciences, Engineering and Technology, VIT Bhopal University, Kothrikalan, Madhya Pradesh, India.
Jabez W OsborneSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. jabez.vit@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lead (Pb) contamination severely affects plant growth and ecosystem health. The present study integrates systems biology and metabolomics to elucidate Pb stress tolerance mechanisms in Pennisetum purpureum bioaugmented with Enterobacter cloacae. Network based metabolite-gene mapping identified 31 plant metabolites and 65 microbial metabolites associated with Pb uptake and oxidative stress mitigation. Key compounds such as ascorbic acid, glutamine, enterochelin, and methionine were linked to target genes APX1, GLU1, entF, and metG, highlighting antioxidant and metal chelation roles. Pathway enrichment revealed activation of ascorbate, glutathione, methionine, and siderophore biosynthetic routes. Pot culture experiments confirmed enhanced plant growth and Pb tolerance in bioaugmented systems supplemented with 100 µg L⁻¹ of ascorbic acid and glutamine. Gas chromatography-mass spectrometry (GC-MS) profiling of root exudates validated these findings, detecting unique metabolites such as thiophene-3-ol and benzeneethanamine associated with improved plant vigour. These results provide a mechanistic framework for exploiting multi-biosystem metabolomics in phytoremediation and the design of effective plant-microbe strategies for Pb bioremoval.

Indexed as

Enterobacter cloacaeLeadPennisetumStress, PhysiologicalBiodegradation, EnvironmentalGas Chromatography-Mass SpectrometryMetabolomicsOxidative StressPlant RootsSystems BiologyLeadBioaugmentationBiosystemsHUB genesMetabolite networkingRhizosphere community

Identifiers

PMID41236546

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.