Evidence map›Paper›PMID 41234633›Full record

ArticleBiochemistry and biophysics reports2025

Characterization of different isogenic mutants derived from improved strains of

O G Ndochinwa, Qing-Yan Wang, O C Amadi, T N Nwagu, C I Nnamchi, A N Moneke

Abstract read
In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

O G NdochinwaDepartment of Microbiology Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
Qing-Yan WangState Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China.
O C AmadiDepartment of Microbiology Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
T N NwaguDepartment of Microbiology Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
C I NnamchiDepartment of Microbiology Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
A N MonekeDepartment of Microbiology Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to improve the thermal and pH stability of pullulanase enzymes through protein engineering. Pullulanase is crucial in various industries, including food, detergent, and textile, as it breaks down starch into simple sugars. Using the active hydrogen bond network (AHBN) method, researchers constructed isogenic mutants of the

Indexed as

Active hydrogen bond networkHydrolysisPullulanase

Identifiers

PMID41234633
PMCPMC12605932

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.