Evidence map›Paper›PMID 41234227›Full record

ArticleFrontiers in endocrinology2025

Identification and validation of key mitophagy-related biomarkers in the pathogenesis of proliferative diabetic retinopathy.

Yifan Liu, Ye Zhou, Jie Chen, Jiahui Jin, Xueying Wang, Xi Wang, Jieping Zhang, Jiao Li, Junfang Zhang, Ling Zhu and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yifan LiuDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Ye ZhouDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Jie ChenDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Jiahui JinDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Xueying WangDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Xi WangDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Jieping ZhangDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Jiao LiDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Junfang ZhangDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Ling ZhuDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Guo-Tong XuDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Yanlong BiDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Qingjian OuDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Caixia JinDepartment of Ophthalmology and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus, and proliferative diabetic retinopathy (PDR) represents its advanced stage. The etiology of PDR is complex. Mitophagy, the selective degradation of dysfunctional mitochondria, is crucial for cellular homeostasis and has been implicated in PDR pathogenesis. However, its specific mechanisms require further investigation. Materials and methods: Gene Expression Omnibu (GEO) datasets (GSE102485, GSE60436) were analyzed in R software to identify differentially expressed mitophagy-related genes (DEMRGs). A PDR diagnostic model was constructed by gene ontology (GO) enrichment analysis, genome enrichment analysis (GSEA), and other relevant methods. Immune infiltration was also performed to analyze the changes in immune cells. Finally, the retinal pigment epithelial cell line (ARPE-19) was incubated with high glucose (HG) to simulate a DR model Results: Eight DEMRGs were identified enabling construction of a PDR diagnostic model and prioritization of two hub genes (CASP8 and COL1A1). Finally, qRT-PCR, Western blotting, and IF were performed to provide preliminary validation of the PDR model and HG stimulation increased mitochondria-lysosome colocalization as well as enhanced the expression of mitophagy-related proteins. Conclusion: Integrated bioinformatics and experimental validation suggest that mitophagy contributes to PDR pathogenesis. Five DEMRGs showed up-regulated and immune cell infiltration that may affect the occurrence and PDR development by regulating mitophagy. These findings provide candidate biomarkers and mechanistic insight into PDR.

Indexed as

BiomarkersDiabetic RetinopathyMitophagyCell LineHumansMitochondriaRetinal Pigment EpitheliumBiomarkersCASP8COL1A1differentially expressed genesmitophagyproliferative diabetic retinopathy

Identifiers

PMID41234227
PMCPMC12605334

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.