ArticleScientific reports2025
Effects of ultra-fast nanosecond electric pulses on mitochondria transmembrane potential and oxidation.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
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Authors and funding
8 authors.
Funding
Abstract
Electroporation can be successfully employed for controlled molecular delivery and therefore has found clinical applications for treatment of cancer. However, it’s a pulse-dependent phenomenon, thus modulation of the effects is possible by developing new parametric protocols for pulsed electric field generation. In this work, we have developed a generator capable of generating 50 ns pulses with extreme pulse repetition frequency (up to 6.6 MHz), which should enable plasma membrane permeabilization at significantly lower electric field thresholds due to burst compression and modulation of intracellular effects specific to nanosecond range. We have investigated the effects of 6–16 kV/cm, 50 and 300 ns pulses on mitochondria depolarization, followed by ATP depletion study and characterization of mitochondria oxidation. Finally, we have experimentally confirmed the feasibility of the proposed nanosecond pulsed electric field bursts for calcium electrochemotherapy in vitro. For consolidation of knowledge, we have included the results of standard microsecond pulse procedures (8 × 100 µs). As model a CHO-K1-Luc cell line was used. Based on the experimental data, it is concluded that nanosecond pulses (50 ns) when delivered at ultra-fast repetition frequency allow reduction of cell membrane permeabilization thresholds and can be successfully used for calcium electrochemotherapy even with PEF amplitudes as low as 10 kV/cm.
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Registered trials
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