ArticleNature communications2025
Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A One-Step Chemoselective Strategy for Hydroxymethylcytosine Sequencing in DNA and RNA.bioRxiv : the preprint server for biology · 2026Article
- Article
- Machine learning-assisted analysis of saliva-containing mixtures using a saliva-specific multi-CpG-microhaplotype marker system.International journal of legal medicine · 2026Article
- Multi-omics-driven precision medicine.iMeta · 2026Review
- Fluorescent Analysis of KBiomolecules · 2026Article
- The potential of methylation signal detection in eDNA toward functional ecological monitoring.Communications biology · 2026Review
- Ultra-mild bisulphite sequencing for DNA methylation analysis from low-input clinical specimens.Clinical epigenetics · 2026Article
- LEMONmethyl-seq: Targeted long-read DNA methylation profiling reveals dynamics of CRISPR epigenome editing and endogenous DNA methylation patterns.bioRxiv : the preprint server for biology · 2026Article
- Methylation profiling in neuropathological tumors diagnosis: a comprehensive review.Frontiers in oncology · 2025Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlights its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.
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Registered trials
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