ArticleOrganogenesis2025
Mechanism of CGF in osteogenic differentiation of periodontal ligament stem cells through the Wnt pathway.
Article in Organogenesis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Concentrated growth factor in oral bone regeneration: A narrative review.Regenerative therapy · 2026Review
- miR-181d-5p promotes valvular calcification and directly targets Wnt inhibitory factor 1 in calcific aortic valve disease.Molecular and cellular biochemistry · 2026Article
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Authors and funding
4 authors.
Funding
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Abstract
objectivePeriodontal ligament stem cells (PDLSCs), undifferentiated mesenchymal cells with multipotent differentiation and self-renewal capacities, constitute the optimal MSC population for periodontal regeneration. This study sought to elucidate the effects of concentrated growth factor (CGF) on the osteogenic differentiation of PDLSCs and analyze the underlying mechanisms.
methodsPDLSCs were isolated from the molars of patients with malocclusion and characterized by flow cytometry, osteogenic induction, lipogenic induction, ARS staining and ORO staining. PDLSCs were treated with osteogenic induction medium containing different concentrations of CGF. The osteogenic ability of CGF in PDLSCs was analyzed via ALP staining, ARS staining, and ALP activity assays. WNK1, RUNX2 and OPN were detected by RT-qPCR. WNK1, RUNX2, OPN,
resultsPDLSCs were successfully isolated and cultured in vitro. After CGF treatment, ALP activity, mineralization nodule formation, and the expression of RUNX2 and OPN in PDLSCs were increased, with 0.1 mg/mL CGF showing the best osteogenic differentiation ability. WNK1,
conclusionCGF promotes PDLSC osteogenic differentiation by activating the Wnt pathway through WNK1.
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