Evidence map›Paper›PMID 41231748›Full record

ArticleNeonatology2026

Inhibitory Potency of a Plant-Based Zinc Protoporphyrin on Heme Oxygenase Activity.

Lucie Muchová, G S Kiran Kumar Reddy, Grant S Wells, Hui Zhao, Hendrik J Vreman, Ronald J Wong, David K Stevenson

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Article in Neonatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Lucie MuchováInstitute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Prague, Czechia.
G S Kiran Kumar ReddySeqens CDMO, Devens, Massachusetts, USA.
Grant S WellsStanford Maternal and Child Health Research Institute, Stanford University School of Medicine, Stanford, California, USA.
Hui ZhaoDepartment of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.
Hendrik J VremanDepartment of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.
Ronald J WongDepartment of Pediatrics, Stanford University School of Medicine, Stanford, California, USA, rjwong@stanford.edu.
David K StevensonDepartment of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

<p>Introduction: Metalloporphyrins, competitive heme oxygenase (HO) inhibitors, may potentially be used as drugs for preventing neonatal hyperbilirubinemia. Metalloporphyrins that specifically target the inducible HO-1 without inhibiting the constitutive HO-2 are the most ideal. Zinc protoporphyrin (ZnPP) has the most promise. We have derived a plant-based ZnPP (ZnPP-Plant) and evaluated its inhibitory potency and selectivity for the HO-1 isozyme.

methodsEleven-µM ZnPP-Plant or technical grade ZnPP (ZnPP-TG) were added to reaction vials containing heme, NADPH, and adult mouse spleen, brain, and liver sonicates. Gas chromatography was used to measure total in vitro HO activity in sonicates. Percent inhibition of control HO activity was then compared.

resultsAt a 11-µM concentration, ZnPP-Plant and ZnPP-TG inhibited HO activity in the liver (69.7 ± 9.3% and 74.2 ± 10.3%, respectively); spleen (65.8 ± 17.9% and 46.8 ± 8.7%, respectively); and brain (54.1 ± 13.3%, and 38.1 ± 13.9%, respectively).

conclusionZnPP-Plant has equal inhibitory potency as ZnPP-TG, and thus has potential use for treating neonatal hyperbilirubinemia. </p>.

Indexed as

Enzyme InhibitorsHeme Oxygenase-1Heme Oxygenase (Decyclizing)ProtoporphyrinsAnimalsBrainHyperbilirubinemia, NeonatalLiverMiceSpleenEnzyme InhibitorsHeme Oxygenase-1Heme Oxygenase (Decyclizing)Protoporphyrinszinc protoporphyrinBilirubinHyperbilirubinemiaJaundiceMetalloporphyrinPhototherapy

Identifiers

PMID41231748
PMCPMC12695104

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.