Evidence map›Paper›PMID 41231271›Full record

ArticlePlant cell reports2025

A VIGS-based leaf disc method reveals ethylene's crucial role in hydrangea leaf spot resistance.

Xintong Liu, Huijie Chen, Shuangshuang Chen, Jing Feng, Xiangyu Qi, Zhenying Wen, Muhammad Zulfiqar Ahmad, Ming Sun, Yan Dong, Yanming Deng

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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Establishment of a TRV-VIGS system in Coix (Frontiers in plant science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xintong LiuInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Huijie ChenInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Shuangshuang ChenInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Jing FengInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Xiangyu QiInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Zhenying WenInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Muhammad Zulfiqar AhmadInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Ming SunState Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing, 100083, China.
Yan DongChina Flower Association, Beijing, 100102, China.
Yanming DengInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. dengym@jaas.ac.cn.ORCID http://orcid.org/0000-0001-7978-1417

Funding

Foundation of Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration KFE202401the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province CX (23) 3062
6 · The paper itself

Abstract

The bigleaf hydrangea (Hydrangea macrophylla) is an emerging fashionable flower. However, leaf spot disease caused by Corynespora cassiicola is a major fungal disease in this species, seriously limiting the high-quality development of the industry. Since potential C. cassiicola induced genes have been identified via RNA-seq data, we aimed to develop an efficient method to screen C. cassiicola resistant genes in hydrangea. Firstly, we established a C. cassiicola inoculation system for detached leaf discs (CISDLD) and applied it to assess leaf spot resistance in hydrangea cultivators. Then, HmPDS1 and HmPDS2 were selected as the reporter genes to test the silencing effect in both cuttings and detached leaf discs, through observing a significant reduction in gene expression and chlorophyll content. Furthermore, ethylene was identified as a positive regulator in hydrangea defense responding to C. cassiicola, as well as jasmonic acid. The results showed that ethylene synthesis genes (HmACS1 and HmACO3) and signaling transduction genes (HmEIN3 and HmERF001) were validated as the C. cassiicola resistant genes in hydrangea. The VIGS screen of C. cassiicola-induced ethylene related genes demonstrated the potential benefits of this method for the high-throughput identification of gene function. This study offers a rapid approach for characterizing C. cassiicola-related gene functions in hydrangea and provides a theoretical framework for high-throughput gene screening in other plant species. KEY MESSAGE: A rapid and effective method for gene function screening based on VIGS with detached leaf discs reveals the crucial role of ethylene in hydrangea leaf spot resistance.

Indexed as

AscomycotaDisease ResistanceEthylenesPlant DiseasesPlant LeavesCyclopentanesGene Expression Regulation, PlantOxylipinsPlant Growth RegulatorsPlant ProteinsCyclopentanesethyleneEthylenesjasmonic acidOxylipinsPlant Growth RegulatorsPlant ProteinsCorynespora cassiicolaEthyleneGene function screeningHydrangea macrophyllaVirus-induced gene silencing

Identifiers

PMID41231271

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.