ArticleTurkish journal of biology = Turk biyoloji dergisi2025
hsa_circ_0001776 regulates the progression of acute myeloid leukemia through the miR-1269b/PTEN axis.
Article in Turkish journal of biology = Turk biyoloji dergisi, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background/aim: Acute myeloid leukemia (AML) is a malignant neoplasm arising from bone marrow hematopoietic stem cells. It is a common subtype of childhood leukemia and remains challenging to cure. Emerging evidence suggests that circular RNAs (circRNAs), a class of noncoding RNAs, play key regulatory roles in tumor biology. Among their various functions, circRNAs often act as 'sponges' for microRNAs (miRNAs), modulating gene expression posttranscriptionally. This study investigates the functional role and clinical relevance of hsa_circ_0001776 in AML. Materials and methods: Three AML cell lines and 22 peripheral blood samples were analyzed. Differential expression analysis of circRNAs in a GSE dataset was performed to identify significantly down- and upregulated candidates, with thresholds set at logFC less than -1 and p < 0.05 for downregulation, and logFC more than 1 and p < 0.05 for upregulation. The back-splice junction of hsa_circ_0001776 was validated using Sanger sequencing. Its circular nature and stability were confirmed via actinomycin D treatment and RNase R digestion. Quantitative real-time PCR (qRT-PCR) was used to measure circRNA levels in clinical AML samples. The functional effects of hsa_circ_0001776 on proliferation and cell cycle progression were evaluated using the CCK-8 assay and flow cytometry. Bioinformatics analyses predicted putative miRNA interactions, which were validated by dual luciferase reporter assays. A p-value less than 0.05 was considered statistically significant. Results: hsa_circ_0001776 expression was significantly reduced in AML samples. Overexpression of hsa_circ_0001776 inhibited cell proliferation and induced G1 phase arrest in AML cells, whereas knockdown of hsa_circ_0001776 accelerated cell cycle progression and promoted malignant proliferation. hsa_circ_0001776 was shown to interact with miR-1269b, the pair being negatively correlated. Conclusion: hsa_circ_0001776 suppresses AML progression via the miR-1269b/
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