Evidence map›Paper›PMID 41228556›Full record

ArticleNutrients2025

Neurodevelopmental Changes in the Guinea Pig Brain Caused by Time-Limited Complete Vitamin C Deprivation.

Ivan Čapo, Ilija Andrijević, Nataša Čapo, Milan Popović, Ivan Milenković, Radomir Ratajac, Dejan Vranješ, Dragana Milutinović, Dragana Simin, Slobodan Sekulić

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Article in Nutrients, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Ivan ČapoDepartment of Histology and Embryology, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0001-7531-5099
Ilija AndrijevićDepartment of Nursing, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0001-8668-3946
Nataša ČapoDepartment for Epidemiology, Faculty of Medicine, Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0009-0006-7031-7398
Milan PopovićDepartment of Histology and Embryology, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0002-7184-3393
Ivan MilenkovićDepartment of Neurology, Medical University of Vienna, 1090 Vienna, Austria.ORCID 0000-0002-2990-0243
Radomir RatajacDepartment for Food Safety and Drug Analysis, Scientific Veterinary Institute "Novi Sad", 21000 Novi Sad, Serbia.ORCID 0000-0002-3165-4176
Dejan VranješFaculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0009-0004-5215-3938
Dragana MilutinovićDepartment of Nursing, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0003-2693-543X
Dragana SiminDepartment of Nursing, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0002-2595-894X
Slobodan SekulićDepartment of Neurology, Faculty of Medicine Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.ORCID 0000-0001-7889-310X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

objectivesThe guinea pig is a unique experimental model because of the evolutionary loss of the GULO gene, which encodes an enzyme involved in vitamin C synthesis. Since vitamin C plays an essential role in collagen biochemistry, numerous studies have investigated the effects of pre- and postnatal vitamin C deficiency. However, only a few studies, including ours, have indicated a possible link between vitamin C deprivation and potential weakening of the basement membrane, which may lead to significant alterations in brain structure.

methodsThe experiment included guinea pig foetuses completely deprived from the 10th (E2 group) and the 20th (E1 group) to the 50th day of intrauterine life. Tissue samples from the cerebrum and cerebellum were taken for biochemical, molecular, and immunohistochemical analyses.

resultsIn the E2 group alone, we found marked gross changes: cerebral bleeding, porencephaly, and a lissencephalic cerebellar surface. Microscopic examination revealed diffuse bleeding in the cerebrum along with a loss of neurons in the area of the defect, specifically in the E2 group. The complete maturation of ectopic neurons characterised dysplastic changes in the cerebellum. Hydroxyproline analysis of both the cerebrum and cerebellum showed no significant differences among the E1, E2, and control groups. However, decreased expression of COL1, COL4A1, and SLC23A1 was observed solely in the cerebellar tissue of the E1 group.

conclusionsThe morphological, biochemical, and molecular results represent preliminary associations with vitamin C deficiency, but require further validation.

Indexed as

Ascorbic AcidAscorbic Acid DeficiencyBrainAnimalsCerebellumCerebrumCollagen Type ICollagen Type IVDisease Models, AnimalFemaleGuinea PigsPregnancySodium-Coupled Vitamin C TransportersAscorbic AcidCollagen Type ICollagen Type IVSodium-Coupled Vitamin C Transportersascorbic acidbraindeficiency diseasesguinea pigneuropathology

Identifiers

PMID41228556
PMCPMC12611027

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