Aggregation-Induced Emission-Fluorescent-Microsphere-Based Lateral Flow Immunoassay for Highly Sensitive Detection of Capsaicinoids.

Yuchen Bai, Xinyue Han, Yang Yang, Zhanhui Wang, Fubin Qiu

Abstract read

In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Yuchen BaiMOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Department of Nutrition and Food Hygiene, College of Public Health, Shanxi Medical University, Taiyuan 030001, China.ORCID 0009-0008-6420-0884

Xinyue HanMOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Department of Nutrition and Food Hygiene, College of Public Health, Shanxi Medical University, Taiyuan 030001, China.

Yang YangMOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Department of Nutrition and Food Hygiene, College of Public Health, Shanxi Medical University, Taiyuan 030001, China.ORCID 0009-0006-4752-8779

Zhanhui WangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.ORCID 0000-0002-0167-9559

Fubin QiuMOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Department of Nutrition and Food Hygiene, College of Public Health, Shanxi Medical University, Taiyuan 030001, China.

Funding

the Basic Research Plan of Shanxi Province No. 202203021222260the National Science Foundation of China No. 82404321

6 · The paper itself

Abstract

Capsaicinoids (CPCs) are regarded as a typical marker of waste oil, which has emerged as a serious food safety issue in developing countries, necessitating the development of rapid, sensitive, and specific detection methods. In this study, a novel hapten was synthesized to generate a high-affinity monoclonal antibody (mAb) targeting CPCs. Subsequently, aggregation-induced emission fluorescent microspheres (AIEFMs), known for their superior fluorescence intensity, were utilized as an enhanced probe to develop a lateral flow immunoassay (LFIA) based on mAb 8B4 for CPC detection. For comparison, a traditional gold nanoparticle (AuNP)-LFIA was also constructed using the corresponding mAb. The AIEFM-LFIA demonstrated a limit of detection (LOD) of 0.33 µg/kg for CPCs in edible oil samples, which is 4.21 times lower than the LOD of 1.39 µg/kg achieved by the AuNP-LFIA. And the assay effectively identified three additional CPCs, with LODs ranging from 0.26 to 0.99 µg/kg, while exhibiting minimal cross-reactivity with CPC analogs, indicating high specificity. The recovery rates of the AIEFM-LFIA in oil samples ranged from 75.0% to 106.0%, with coefficients of variation ≤ 8.3%, exhibiting excellent accuracy and precision. Furthermore, the results of the AIEFM-LFIA demonstrated a strong degree of correlation with liquid chromatography-tandem mass spectrometry, with a correlation coefficient (R

Indexed as

aggregation-induced emissioncapsaicinoidslateral flow immunoassaysensitive detectionwaste oil

Identifiers

PMID41227608

DOI10.3390/foods14213634

PMCPMC12608905

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