Evidence map›Paper›PMID 41227474›Full record

ArticleAnimals : an open access journal from MDPI2025

Construction of a Duck Intestinal Organoid Culture System: From Crypt Isolation to Medium Optimization.

Rui Tang, Xiang Luo, Li Zhang, Zhenhua Liang, Yan Wu, Jingbo Liu, Jinsong Pi, Hao Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rui TangHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-4890-459X
Xiang LuoHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-5172-4485
Li ZhangHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-4397-1785
Zhenhua LiangHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-1442-546X
Yan WuHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0001-9559-8583
Jingbo LiuSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.ORCID 0009-0006-6499-4499
Jinsong PiHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-7293-545X
Hao ZhangHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, No. 1 Nanhuyaoyuan, Nanhu Road, Wuhan 430064, China.ORCID 0000-0002-3720-4459

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal organoids possess self-organizing capacity and recapitulate essential features of intestinal architecture and function, making them powerful models for investigating development, disease mechanisms, pharmacological testing, and host-microbe interactions. Although standardized protocols for chicken intestinal organoids have been established, a defined culture system for ducks has not been available. In this study, we optimized crypt isolation procedures and culture medium composition to establish a reproducible system tailored to duck intestinal stem cells. Among various digestive solutions, ethylene glycol tetraacetic acid (EGTA) achieved the highest crypt isolation efficacy and organoid survival. Suspension culture resulted in better survival, proliferation, and differentiation of intestinal stem cells than air-liquid interface and embedding methods (

Indexed as

cryptsculture systemduckintestinal organoids

Identifiers

PMID41227474
PMCPMC12607406

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.