Evidence map›Paper›PMID 41226739›Full record

ArticleInternational journal of molecular sciences2025

Comparative Analysis of CRISPR/Cas9 Delivery Methods in Marine Teleost Cell Lines.

Álvaro J Arana, Sara Veiga-Rua, Diego Cora, Manuel A Gónzalez-Gómez, Ana Seijas, Maialen Carballeda, David Polo, Alberto Cuesta, Yolanda Piñeiro, José Rivas and 5 more

Abstract readComparative Study
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Advances in Fish Gene Editing.Animals : an open access journal from MDPI · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Álvaro J AranaDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0002-9857-3469
Sara Veiga-RuaDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0002-2363-2815
Diego CoraDepartamento de Química Física, Facultade de Ciencias, Campus Terra, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
Manuel A Gónzalez-GómezNANOMAG Laboratory, Department of Applied Physics, Materials Institute (iMATUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.ORCID 0000-0002-6234-228X
Ana SeijasDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
Maialen CarballedaDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0009-0007-9085-1704
David PoloNanoToxGen Group, CICA-Centro Interdisciplinar de Química e Bioloxía, Universidade da Coruña, Rúa As Carballeiras, 15071 A Coruña, Spain.ORCID 0000-0002-0842-003X
Alberto CuestaFish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.ORCID 0000-0002-0906-7995
Yolanda PiñeiroNANOMAG Laboratory, Department of Applied Physics, Materials Institute (iMATUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
José RivasNANOMAG Laboratory, Department of Applied Physics, Materials Institute (iMATUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.ORCID 0000-0002-5059-3196
Mercedes NovoDepartamento de Química Física, Facultade de Ciencias, Campus Terra, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0002-4869-289X
Wajih Al-SoufiDepartamento de Química Física, Facultade de Ciencias, Campus Terra, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0003-2535-8546
Paulino MartínezDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0001-8438-9305
Laura SánchezDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.ORCID 0000-0001-7927-5303
Diego RobledoDepartamento de Zooloxía, Xenética e Antropoloxía Física, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.

Funding

Agencia Estatal de Investigación 10.13039/501100011033European Union ERC Starting Grant programme 2022 No 101076432 (FishTRIM)Fundación Caixa Rural Galega Tomás Notario Vacas Optimizing CRISPR/Cas9 genome editing to improve disease resistance in aquacultureMinisterio de Ciencia, Innovación y Universidades RYC2023-044793-IOportunius programme of the Axencia Galega the Innovación (GAIN, Xunta de Galicia) (BBS/E/20002172, BBS/E/D/30002275, BBS/E/D/10002070 and BBS/E/RL/230002A)Spain's Recovery and Resilience Plan investment line nº 1 and component number 17, which includes the Complementary RTDI Plan for Marine ScienceUniversidade de Santiago de Compostela CRISPR-NANOXEN
6 · The paper itself

Abstract

Gene editing technologies such as CRISPR/Cas9 have revolutionized functional genomics, yet their application in marine fish cell lines remains limited by inefficient delivery. This study compares three delivery strategies-electroporation, lipid nanoparticles (LNPs), and magnetofection using gelatin-coated superparamagnetic iron oxide nanoparticles (SPIONs)-for CRISPR/Cas9-mediated editing of the

Indexed as

CRISPR-Cas SystemsFishesGene EditingGene Transfer TechniquesAnimalsCell LineElectroporationTransfectionaquacultureCas9CRISPRediting efficiencygene editingsea basssea bream

Identifiers

PMID41226739
PMCPMC12610623

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.