Utilization of All-Chitin Composite Films for High-Density Three-Dimensional Cell Cultivation.

Masayasu Totani, Mako Eda, Hiroyuki Shinchi, Jun-Ichi Kadokawa

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Construction and Advanced Utilization of Self-Assembled and Scale-Down Chitin Nanofibers for Polymer Composite Design.Molecules (Basel, Switzerland) · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Masayasu TotaniGraduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.ORCID 0000-0001-6471-3622

Mako EdaGraduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.

Hiroyuki ShinchiGraduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.ORCID 0000-0001-5204-9170

Jun-Ichi KadokawaGraduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.ORCID 0000-0001-8813-1950

Funding

Japan Society for the Promotion of Science 24K08513

6 · The paper itself

Abstract

In this study, the potential of an all-chitin composite (AChC) film, prepared by dispersing and mixing high-crystalline scaled-down chitin nanofibers (SD-ChNFs) and low-crystalline chitin nanoparticles (ChNPs) in aqueous acetic acids, was evaluated for the formation of tissue-like cellular networks using a layered culture approach. The AChC film exhibited strong adhesion properties that enabled human cervical cancer (HeLa) cells to form a cellular network structure, whereas such adhesion and network formation were not observed with the individual components, SD-ChNFs and ChNPs. Furthermore, the cellular network structure was found to bridge the gaps between films, establishing three-dimensional (3D) connectivity. These findings demonstrate that AChC films serve as highly suitable functional biomaterials for constructing 3D tissue-like structures.

Indexed as

Cell Culture Techniques, Three DimensionalChitinNanofibersBiocompatible MaterialsCell AdhesionHeLa CellsHumansNanoparticlesTissue EngineeringBiocompatible MaterialsChitinall-chitin compositecell morphologycell network formationthree-dimensional cell cultivation

Identifiers

PMID41226203

DOI10.3390/molecules30214243

PMCPMC12608466

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.