Evidence map›Paper›PMID 41226195›Full record

ArticleMolecules (Basel, Switzerland)2025

Effects of Swapping 5HT3 and α7 Residues in Chimeric Receptor Proteins on RIC3 and NACHO Chaperone Actions.

Zixuan Yan, Sreeharshini Oruganti, Swetha K Iyer, Kushboo Agarwal, Mitali Gupta, Ketaki Adhikari, Nevetha Vijayan, Jash Doda, Vaishali Jain, Arya N Lokhande and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Zixuan YanDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Sreeharshini OrugantiDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.ORCID 0009-0009-7333-3811
Swetha K IyerDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Kushboo AgarwalDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Mitali GuptaDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Ketaki AdhikariDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Nevetha VijayanDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Jash DodaDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.ORCID 0009-0009-7403-9825
Vaishali JainDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Arya N LokhandeDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Aadhya P NairDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Venkat Sri K NallanichakravarthulaDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Maegan M WeltzinDepartment of Chemistry and Biochemistry, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775, USA.ORCID 0000-0001-9051-8150
Ralph H LoringDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.ORCID 0000-0002-9228-4981

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alpha7 nicotinic receptors (α7-nAChRs) are implicated in many neurological disorders, but how they fold and assemble is not well understood. Unlike native α7-nAChRs, α7-5HT3 chimeras fold efficiently in HEK cells and do not require chaperones RIC3 or TMEM35A (NACHO) for proper assembly. We investigated the effects of swapping 5HT3 and α7-receptor protein sequences on α7-5HT3R chimera surface expression in mammalian HEK293 or Bosc23 cells, or chimeric receptor function using

Indexed as

alpha7 Nicotinic Acetylcholine ReceptorMolecular ChaperonesReceptors, Serotonin, 5-HT3Recombinant Fusion ProteinsAnimalsHEK293 CellsHumansIntracellular Signaling Peptides and ProteinsMiceOocytesXenopus laevisalpha7 Nicotinic Acetylcholine ReceptorIntracellular Signaling Peptides and ProteinsMolecular ChaperonesReceptors, Serotonin, 5-HT3Recombinant Fusion ProteinsRIC3 protein, humanalpha-bungarotoxin bindingchaperone actionsImageJ analysisquantitative fluorescent binding assayreceptor assemblyreceptor subunit foldingtransmembrane domains

Identifiers

PMID41226195
PMCPMC12608704

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.