ArticleBMC plant biology2025
Transcriptome-wide analysis of AlbHLH gene family in Atractylodes lancea (Thunb.) DC and functional exploration of AlbHLH3 in regulating terpenoid biosynthesis.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Chromosome-scale genome assembly and genomic prediction of essential oil compounds in Atractylodes lancea for genomics-assisted breeding.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Article
- Characterization of AP2/ERF and bHLH transcription factors and association with terpenoid metabolism inFrontiers in plant science · 2026Article
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Abstract
backgroundAtractylodes lancea (Thunb.) DC., a medicinal species of Asteraceae with multi-pharmacological applications in rheumatic disorders and gastrointestinal syndromes due to its bioactive terpenoid-enriched essential oils. Although basic helix-loop-helix (bHLH) transcription factors are well known regulators of secondary metabolism biosynthesis pathways, their functional characterization in terpenoid regulation in A. lancea remains unexplored.
results176 AlbHLHs were systematically identified through multi-tissue transcriptomic profiling (leaf, stem, rhizome, rhizome buds) and categorized them into 21 evolutionarily conserved subfamilies via phylogenetic analysis with Arabidopsis thaliana bHLH proteins. Conserved motif architecture revealed high intra-subfamily conservation. Transcriptome analysis revealed that 36 AlbHLHs with significant differential expression across tissues, 19 of exhibited highly expressed in rhizomes and rhizome buds, which consistent with the accumulation trend of terpenoids determined by GC-MS analysis. Moreover, yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA) confirmed that AlbHLH3 could interact with the promoters of the key rate-limiting enzyme in terpenoid biosynthesis pathway genes 3-hydroxy-3-methylglutaryl-CoA reductase (AlHMGR3). Transient overexpression of AlbHLH3 significantly upregulated expression of not only AlHMGR3 but also downstream terpenoid pathway genes (AlMVK, AlMVD, AlPMK), suggesting its regulatory roles in terpenoid biosynthesis.
conclusionsThis study contributes significantly to the understanding of the regulatory role of AlbHLHs in terpenoid biosynthesis and provides potential possibilities genetic modification strategies to enhance the medicinal value of A. lancea.
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