ArticleNature food2025
Spontaneous immortalization of bovine fibroblasts following long-term expansion offers a non-transformed cell source for cultivated beef.
Article in Nature food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.Biotechnology and bioengineering · 2026Review
- Stem Cells for Cultured Meat: Cell Sources, Lineage Specification, and Biomaterial Scaffolds for Edible Tissue Engineering.International journal of molecular sciences · 2026Review
- Comparing meat alternatives for a sustainable food system.NPJ science of food · 2026Article
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Safety Aspects of Cell Culture-Derived Food for Human Consumption.Advances in biochemical engineering/biotechnology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spontaneously immortalized cell lines provide an essential, non-transformed resource for cultivated meat production. Although chicken fibroblasts readily immortalize in culture, bovine fibroblasts have not been shown to immortalize without genetic manipulation of TP53 or TERT. Here we demonstrate the spontaneous immortalization of fibroblast lines from Simmental and Holstein cows. We track the molecular basis of the immortalization process over 500 days of culture, corresponding to 240 population doublings. Cells entered senescence at population doubling 60, showing γH2AX foci, telomere shortening and an active senescence-associated secretory phenotype profile. Breakthroughs occurred following 400 days in culture, resulting in stable fibroblast lines. Telomerase and PGC1A activation during senescence resolve telomere shortening and mitochondrial dysfunction without activating P53, driving spontaneous immortalization. We explored the economic potential of cultivated beef production using spontaneously immortalized bovine fibroblasts, showing that price parity could be theoretically reached using continuous manufacturing.
Indexed as
Identifiers
41225077What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.